Wisniewski H M, Merz P A, Iqbal K
J Neuropathol Exp Neurol. 1984 Nov;43(6):643-56. doi: 10.1097/00005072-198411000-00008.
Isolated paired helical filaments (PHF) of Alzheimer's disease and senile dementia of the Alzheimer type (AD/SDAT) were studied by negative stain techniques and electron microscopy (EM). The helical structure of the PHF was confirmed by tilt analysis of negatively stained isolated PHF. A substructure of four protofilaments, each 3-5 nm in diameter, was also observed. A comparison of PHF substructure, neurofilaments and microtubules, revealed no similarity among the three structures.
采用负染技术和电子显微镜（EM）对阿尔茨海默病及阿尔茨海默型老年痴呆症（AD/SDAT）的分离配对螺旋丝（PHF）进行了研究。通过对负染分离的PHF进行倾斜分析，证实了PHF的螺旋结构。还观察到一种由四条原丝组成的亚结构，每条原丝直径为3 - 5纳米。对PHF亚结构、神经丝和微管的比较表明，这三种结构之间没有相似性。
