Niemelä O, Israel Y
Department of Clinical Chemistry, University of Oulu, Finland.
Lab Invest. 1992 Aug;67(2):246-52.
Previous observations have indicated that acetaldehyde can bind irreversibly to proteins in vitro, yielding immunogenic determinants, which can stimulate production of antibodies against the acetaldehyde adducts.
We have developed sensitive two-site enzyme-linked immunosorbent assays for measurement of hemoglobin-acetaldehyde adducts. These adducts were measured from the red blood cells of 169 alcohol abusers, 66 social drinkers, 18 abstainers, and 73 hospitalized control patients.
While the immunoreactive acetaldehyde adducts were found to be increased in 50% of the alcohol abusers (p less than 0.01), 24% of the social drinkers (p less than 0.05) also exceeded the reference interval obtained from the abstaining controls. Adducts were also increased in 17 (23%) hospitalized controls, seven of whom could retrospectively be verified as heavy drinkers. Upon abstinence from ethanol, the adducts decreased during a period of 1-3 weeks.
The studies indicate that acetaldehyde adducts are frequently elevated in the erythrocytes of human alcohol consumers. Measurements of such adducts may prove to be valuable in the early identification of excessive alcohol consumption and of hazardous social drinking and in the comprehensive assessment and treatment of patients with alcohol-related diseases.
先前的观察表明，乙醛在体外可与蛋白质不可逆结合，产生免疫原性决定簇，进而刺激针对乙醛加合物的抗体产生。
我们开发了灵敏的双位点酶联免疫吸附测定法来检测血红蛋白-乙醛加合物。对169名酗酒者、66名社交饮酒者、18名戒酒者和73名住院对照患者的红细胞进行了这些加合物的检测。
虽然发现50%的酗酒者中免疫反应性乙醛加合物增加（p<0.01），但24%的社交饮酒者（p<0.05）也超过了从戒酒对照者获得的参考区间。17名（23%）住院对照患者的加合物也增加，其中7名可追溯确认为重度饮酒者。戒酒期间，加合物在1至3周内减少。
研究表明，乙醛加合物在人类饮酒者的红细胞中经常升高。检测此类加合物可能在早期识别过度饮酒和有害社交饮酒以及对酒精相关疾病患者的综合评估和治疗中具有重要价值。
