Tanaka M, Hainu M, Yasunobu K T, Norton T R
Biochemistry. 1977 Jan 25;16(2):204-8. doi: 10.1021/bi00621a007.
A highly potent heart stimulant, anthopleurin A, from Anthopleura xanthogrammica was shown to exist as a single polypeptide chain consisting of 49 amino acid residues. The sequence of the peptide was shown to be: Gly-Val-Ser-Cys-Leu-Cys-Asp-Ser-Asp-Gly-Pro-Ser-Val-Arg-Gly-Asn-Thr-Leu-Ser-Gly-Thr-Leu-Trp-Leu-Tyr-Pro-Ser-Gly-Cys-Pro-Ser-Gly-Trp-His-Asn-Cys-Lys-Ala-His-Gly-Pro-Thr-Ile-Gly-Trp-Cys-Cys-Lys-Gln as judged by Edman degradation of the carboxymethylcysteine derivative and the tryptic peptides obtained from the derivative. Although six carboxymethylcysteine residues were present in the polypeptide, no cysteine residues were detectable in the native protein, indicating that there are three cystine residues in anthopleurin A.
一种来自黄斑海葵的高效心脏兴奋剂——海葵毒素A，被证明是由49个氨基酸残基组成的单条多肽链。通过对羧甲基半胱氨酸衍生物进行埃德曼降解以及对从该衍生物获得的胰蛋白酶肽段进行分析，确定该肽的序列为：甘氨酸-缬氨酸-丝氨酸-半胱氨酸-亮氨酸-半胱氨酸-天冬氨酸-丝氨酸-天冬氨酸-甘氨酸-脯氨酸-丝氨酸-缬氨酸-精氨酸-甘氨酸-天冬酰胺-苏氨酸-亮氨酸-丝氨酸-甘氨酸-苏氨酸-亮氨酸-色氨酸-亮氨酸-酪氨酸-脯氨酸-丝氨酸-甘氨酸-半胱氨酸-脯氨酸-丝氨酸-甘氨酸-色氨酸-组氨酸-天冬酰胺-半胱氨酸-赖氨酸-丙氨酸-组氨酸-甘氨酸-脯氨酸-苏氨酸-异亮氨酸-甘氨酸-色氨酸-半胱氨酸-半胱氨酸-赖氨酸-谷氨酰胺。尽管该多肽中存在六个羧甲基半胱氨酸残基，但在天然蛋白质中未检测到半胱氨酸残基，这表明海葵毒素A中有三个胱氨酸残基。
