大鼠背根神经节细胞的逆行标记：荧光金与辣根过氧化物酶标记的定量和形态学比较

Retrograde labelling of dorsal root ganglion cells in the rat: a quantitative and morphological comparison of Fluoro-Gold with horseradish peroxidase labelling.

作者信息

Baranowski A P, Anand U, McMahon S B

机构信息

Department of Physiology, St. Thomas' Hospital Medical School, UMDS, London, UK.

出版信息

Neurosci Lett. 1992 Jul 6;141(1):53-6. doi: 10.1016/0304-3940(92)90332-2.

DOI:10.1016/0304-3940(92)90332-2

PMID:1380681

Abstract

We have compared retrograde labelling of rat primary sensory neurons using Fluoro-Gold (FG) and horseradish peroxide conjugated with wheat germ agglutinin (HRP-WGA). Fluoro-Gold 2.5% after 48 h transit time and FG 5% after 24 and 48 h retrogradely labelled similar numbers of cell profiles as HRP-WGA (P greater than 0.1% Student's t-test). The calculated cell size distribution for the above FG groups were similar to those for the HRP-WGA. However, FG 2.5% after a 24 h transit time labelled significantly fewer cells (P less than 0.001 Student's t-test). FG retrograde transport may be used to identify the same population of DRG cells as HRP-WGA.

摘要

我们比较了使用荧光金（FG）和与麦胚凝集素偶联的辣根过氧化物酶（HRP-WGA）对大鼠初级感觉神经元进行逆行标记的情况。在48小时转运时间后使用2.5%的荧光金以及在24小时和48小时后使用5%的荧光金进行逆行标记的细胞轮廓数量与HRP-WGA相似（学生t检验，P大于0.1%）。上述FG组计算出的细胞大小分布与HRP-WGA组相似。然而，在24小时转运时间后使用2.5%的荧光金标记的细胞明显较少（学生t检验，P小于0.001）。FG逆行运输可用于识别与HRP-WGA相同的背根神经节细胞群体。