荧光金和小麦胚凝集素-辣根过氧化物酶对膀胱传入神经元标记的差异
Differences in Fluorogold and wheat germ agglutinin-horseradish peroxidase labelling of bladder afferent neurons.
作者信息
Kruse M N, Erdman S L, Puri G, de Groat W C
机构信息
Department of Pharmacology, University of Pittsburgh, PA 15261.
出版信息
Brain Res. 1993 Jun 11;613(2):352-6. doi: 10.1016/0006-8993(93)90926-e.
PMID:7514482
Abstract
Rat urinary bladder afferent neurons were significantly smaller (34%) when labelled with Fluorogold (FG) than when labelled with wheat germ agglutinin-horseradish peroxidase (WGA-HRP). This study showed that this difference was due to an artifact of tissue processing (ethanol dehydration) and was not due to uptake and transport of the two tracers by two different subpopulations of bladder afferents.
摘要
用荧光金（FG）标记时，大鼠膀胱传入神经元明显比用麦胚凝集素 - 辣根过氧化物酶（WGA - HRP）标记时小（34%）。本研究表明，这种差异是由于组织处理（乙醇脱水）的假象，而非两种不同的膀胱传入神经元亚群对两种示踪剂的摄取和运输所致。
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