大肠杆菌16S rRNA 530环中的核糖体模糊突变。
A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.
作者信息
O'Connor M, Göringer H U, Dahlberg A E
机构信息
Section of Biochemistry, Brown University, Providence, RI 02912.
出版信息
Nucleic Acids Res. 1992 Aug 25;20(16):4221-7. doi: 10.1093/nar/20.16.4221.
Abstract
A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534.
摘要
在大肠杆菌16S rRNA高度保守的530茎环区域构建了一系列碱基替换和缺失突变，该区域参与tRNA与核糖体A位点的结合。G517的碱基替换和缺失对细胞生长速率和翻译保真度产生了显著影响，使得UGA、UAG和UAA终止密码子能够通读，同时在体内刺激了+1和-1移码。相比之下，534位的突变对生长速率或翻译保真度几乎没有影响。结果表明G517在维持翻译保真度方面的重要性，但不支持G517与U534之间的碱基配对相互作用。
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