大肠杆菌16S rRNA中增强和降低抑制性tRNA活性的突变。
Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.
作者信息
Prescott C D, Kornau H C
机构信息
Max Planck Institut für Molekulare Genetik, Berlin, Germany.
出版信息
Nucleic Acids Res. 1992 Apr 11;20(7):1567-71. doi: 10.1093/nar/20.7.1567.
Abstract
The in vivo expression of mutations constructed within helix 34 of 16S rRNA has been examined together with a nonsense tRNA suppressor for their action at stop codons. The data revealed two novel results: in contrast to previous findings, some of the rRNA mutations affected suppression at UAA and UAG nonsense codons. Secondly, both an increase and a decrease in the efficiency of the suppressor tRNA were induced by the mutations. This is the first report that rRNA mutations decreased the efficiency of a suppressor tRNA. The data are interpreted as there being competition between the two release factors (RF-1 and RF-2) for an overlapping domain and that helix 34 influences this interaction.
摘要
对在16S rRNA螺旋34内构建的突变体的体内表达以及一种无义tRNA抑制子在终止密码子处的作用进行了研究。数据揭示了两个新结果：与先前的发现相反，一些rRNA突变影响了对UAA和UAG无义密码子的抑制作用。其次，这些突变导致抑制子tRNA的效率既增加又降低。这是首次报道rRNA突变会降低抑制子tRNA的效率。这些数据被解释为两个释放因子（RF-1和RF-2）对一个重叠结构域存在竞争，并且螺旋34影响这种相互作用。
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