石膏样毛癣菌部分纯化的细胞外角蛋白酶的一些生化特性

Some biochemical characteristics of a partially purified extracellular keratinase from Trichophyton schoenleinii.

作者信息

Qin L M, Dekio S, Jidoi J

机构信息

Department of Dermatology, Shimane Medical University, Izumo, Japan.

出版信息

Zentralbl Bakteriol. 1992 Jul;277(2):236-44. doi: 10.1016/s0934-8840(11)80618-3.

DOI:10.1016/s0934-8840(11)80618-3

PMID:1381647

Abstract

A Trichophyton schoenleinii (T. schoenleinii) strain from tinea favus was cultured in a liquid medium, from which an extracellular keratinase extract was obtained. The keratinase was partially purified with carboxymethyl-cellulose (CMC) column chromatography. Some biochemical characteristics of the keratinase were then examined. Its molecular weight was estimated to be 38,000 on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The optimal temperature was 50 degrees C, and the optimal pH value was 5.5. The keratinolytic activity was specifically increased by Fe++ and Fe .

摘要

从黄癣中分离出的一株许兰毛癣菌（Trichophyton schoenleinii，T. schoenleinii）在液体培养基中培养，从中获得细胞外角蛋白酶提取物。用羧甲基纤维素（CMC）柱色谱法对角蛋白酶进行部分纯化。然后检测了角蛋白酶的一些生化特性。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上，其分子量估计为38000。最适温度为50℃，最适pH值为5.5。Fe++和Fe能特异性提高角蛋白分解活性。