Singh C J
Department of Botany, St. John's College, Agra, India.
Mycopathologia. 1997;137(1):13-6. doi: 10.1023/a:1006844201399.
The ability of Trichophyton simii HN 50, isolated from the Ghana Bird Sanctuary, Bharatpur, India, to produce extracellular keratinase was studied. Enzyme was produced on a keratin salt broth medium at pH7 and a temperature of 28 +/- 1 degree C. Enzyme secretion was best at 15 days of incubation. Asparagine and keratin were repressive to enzyme yield in comparison to gelatin. No relationship was observed between enzyme release and biomass sugars suppressed keratinase production in descending order as follows: glucose > mannose > maltose > arabinose > fructose. The enzyme showed ability to degrade all of the 3 keratin substrates. Buffalo skin was best degraded in the absence of glucose while chicken feathers were the least degraded in its presence.
对从印度巴拉特普尔加纳鸟类保护区分离出的须癣毛癣菌HN 50产生细胞外角蛋白酶的能力进行了研究。该酶在pH7、温度为28±1℃的角蛋白盐肉汤培养基上产生。酶分泌在培养15天时最佳。与明胶相比，天冬酰胺和角蛋白对酶产量有抑制作用。未观察到酶释放与生物量糖之间的关系，糖对角蛋白酶产生的抑制作用从大到小依次为：葡萄糖＞甘露糖＞麦芽糖＞阿拉伯糖＞果糖。该酶显示出降解所有三种角蛋白底物的能力。在没有葡萄糖的情况下，水牛皮的降解效果最佳，而在有葡萄糖的情况下，鸡毛的降解效果最差。
