Isshiki Y, Haishima Y, Kondo S, Shimada T, Hisatsune K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Microbiol Immunol. 1992;36(6):575-81. doi: 10.1111/j.1348-0421.1992.tb02057.x.
Cross-agglutination and cross-agglutinin absorption experiments were carried out on non-O1 Vibrio cholerae bio-serogroup Hakata (Hakata) and Yersinia enterocolitica O9 (O9). It was shown that the O-antigen of Hakata was closely related to that of O9 in an a, b-a, c type of relationship. The antigenic relationship between the O-antigens of the two bacteria was analyzed by passive hemolysis (PH) and passive hemolysis inhibition (PHI) tests by using their lipopolysaccharides (LPS) as antigen for sensitizing sheep red blood cells (SRBC) and, in the case of the latter, as an inhibitor in a PH system consisting of LPS-coated SRBC, guinea-pig complement and anti-Hakata or O9 antiserum, both unabsorbed and absorbed with the heterologous Hakata or O9 antigen. In the PH experiment, unabsorbed anti-Hakata antiserum had hemolytic titers of 126,100 and 2,600 against Hakata- and O9-LPS-coated SRBC, respectively, and anti-O9 antiserum had hemolytic titers of 19,400 and 38,800, respectively, against these SRBC. The PH experiment showed that anti-O9 antiserum contains a hemolysin reacting with the heterologous Hakata antigen at a high titer (19,400), while anti-Hakata antiserum contains a hemolysin reacting with the heterologous O9 antigen at a significant titer (2,600). The former was completely removed from anti-O9 antiserum with the Hakata antigen and the latter from anti-Hakata antiserum with the O9 antigen. Thus, serological cross-reactivity was demonstrated between the Hakata and O9 strains.
对非O1群霍乱弧菌博多生物血清型(博多)和小肠结肠炎耶尔森菌O9(O9)进行了交叉凝集和交叉凝集素吸收实验。结果表明,博多的O抗原与O9的O抗原在a、b - a、c型关系中密切相关。通过被动溶血(PH)和被动溶血抑制(PHI)试验分析了两种细菌O抗原之间的抗原关系,以它们的脂多糖(LPS)作为抗原致敏绵羊红细胞(SRBC),对于后者,在由LPS包被的SRBC、豚鼠补体和抗博多或O9抗血清组成的PH系统中作为抑制剂,抗血清均未吸收和用异源博多或O9抗原吸收。在PH实验中,未吸收的抗博多抗血清对博多和O9 - LPS包被的SRBC的溶血效价分别为126,100和2,600,抗O9抗血清对这些SRBC的溶血效价分别为19,400和38,800。PH实验表明,抗O9抗血清含有一种与异源博多抗原高滴度(19,400)反应的溶血素,而抗博多抗血清含有一种与异源O9抗原显著滴度(2,6)反应的溶血素。前者用博多抗原从抗O9抗血清中完全去除,后者用O9抗原从抗博多抗血清中完全去除。因此,证明了博多和O9菌株之间存在血清学交叉反应。