Sano Y, Kondo S, Isshiki Y, Shimada T, Hisatsune K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Microbiol Immunol. 1996;40(10):735-41. doi: 10.1111/j.1348-0421.1996.tb01134.x.
Chemical and serological studies were performed with the lipopolysaccharide (LPS) from Vibrio cholerae O144 (O144). The LPS of O144 contained D-glucose, D-galactose, L-glycero-D-manno-heptose, D-fructose, D-quinovosamine (2-amino-2,6-dideoxy-D-gluco-pyranose) and L-perosamine (4-amino-4,6-dideoxy-L-manno-pyranose). The perosamine, a major component sugar of the LPS from O144, was in an L-configuration, as is also the case in the LPS from V. cholerae O76 (O76), in contrast to the D-configuration of the perosamine in the LPS of V. cholerae O1. A structural analysis revealed that the O polysaccharide chain of the LPS from O144 is an alpha(1-->2)-linked homopolymer of (R)-(-)-2-hydroxypropionyl-L-perosamine. The serological cross-reactivity between O144 and O76 was clearly revealed by cross-agglutination and cross-agglutinin absorption tests with whole cells, as well as by passive hemolysis tests with sheep red-blood cells that had been sensitized with the LPS from O144 and O76. In contrast, in passive hemolysis tests, the LPS of O144 did not cross-react serologically with the LPSs from other strains such as V. cholerae O1 (Ogawa and Inaba), V. cholerae O140, Vibrio bio-serogroup 1875 (Original and Variant) and Yersinia enterocolitica O9. The LPSs from these strains consist of O polysaccharide chains composed of alpha(1-->2)-linked homopolymers of D-perosamine with various N-acyl groups, and they share the Inaba antigen factor C of V. cholerae O1 in common. The results obtained in this study demonstrate that the absolute configuration of the perosamine residue in homopolymers plays a very important role in the expression of the serological specificity of the Inaba antigen factor C of V. cholerae O1.
对霍乱弧菌O144（O144）的脂多糖（LPS）进行了化学和血清学研究。O144的LPS含有D-葡萄糖、D-半乳糖、L-甘油-D-甘露庚糖、D-果糖、D-奎诺糖胺（2-氨基-2,6-二脱氧-D-葡萄糖吡喃糖）和L-鼠李糖胺（4-氨基-4,6-二脱氧-L-甘露糖吡喃糖）。鼠李糖胺是O144 LPS的主要组成糖，呈L构型，霍乱弧菌O76（O76）的LPS也是如此，而霍乱弧菌O1 LPS中的鼠李糖胺为D构型。结构分析表明，O144 LPS的O多糖链是（R）-（-）-2-羟基丙酰-L-鼠李糖胺的α（1→2）连接的同聚物。通过全细胞的交叉凝集和交叉凝集素吸收试验，以及用O144和O76的LPS致敏的绵羊红细胞进行的被动溶血试验，清楚地揭示了O144和O76之间的血清学交叉反应性。相比之下，在被动溶血试验中，O144的LPS与其他菌株如霍乱弧菌O1（小川型和稻叶型）、霍乱弧菌O140、弧菌生物血清群1875（原型和变异型）以及小肠结肠炎耶尔森菌O9的LPS没有血清学交叉反应。这些菌株的LPS由O多糖链组成，该多糖链由带有各种N-酰基的D-鼠李糖胺的α（1→2）连接的同聚物组成，并且它们共同具有霍乱弧菌O1的稻叶型抗原因子C。本研究获得的结果表明，同聚物中鼠李糖胺残基的绝对构型在霍乱弧菌O1稻叶型抗原因子C的血清学特异性表达中起着非常重要的作用。
