Zareba G, Chmielnicka J
Department of Toxicological Chemistry, Institute of Environmental Research and Bioanalysis, Medical Academy of Lódź, Poland.
Biol Trace Elem Res. 1992 Aug;34(2):115-22. doi: 10.1007/BF02785241.
The experiment was performed on female rabbits that received per os equimolar doses (17 microM Me/kg) of SnCl2 x 2 H2O or Pb (CH3 COO)2 every day for 5 d. The activity of delta-aminolevulinic acid dehydratase (ALA-D) in the whole blood, liver, kidneys, brain, spleen, and bone marrow, concentration of free erythrocyte protoporphyrins (FEP), activity of delta-aminolevulinic acid synthetase (ALA-S) in the liver and bone marrow, urine delta-aminolevulinic acid (ALA-U), and coproporphyrins (CP-U) were determined. Lead and tin concentrations in the blood were estimated. Lead caused a significant inhibition of ALA-D in the blood, increased FEP concentration, and ALA and CP excretion in urine of rabbits. Lead also decreased ALA-D activity in the bone marrow and in the liver, and did not change ALA-S activity in the liver and bone marrow. Tin did not change any of the examined indices. Tin doses applied in the present study, maintained within the limits of permissible standards of metal levels in human diet, did not affect the process of heme biosynthesis in rabbits.
实验在雌性兔子身上进行，这些兔子每天经口给予等摩尔剂量（17微摩尔/千克体重的二水合氯化亚锡或醋酸铅），持续5天。测定全血、肝脏、肾脏、大脑、脾脏和骨髓中δ-氨基乙酰丙酸脱水酶（ALA-D）的活性、游离红细胞原卟啉（FEP）的浓度、肝脏和骨髓中δ-氨基乙酰丙酸合成酶（ALA-S）的活性、尿δ-氨基乙酰丙酸（ALA-U）和粪卟啉（CP-U）。估算血液中的铅和锡浓度。铅导致兔子血液中ALA-D显著抑制、FEP浓度升高以及尿中ALA和CP排泄增加。铅还降低了骨髓和肝脏中ALA-D的活性，并且未改变肝脏和骨髓中ALA-S的活性。锡未改变任何检测指标。本研究中使用的锡剂量保持在人类饮食中金属水平允许标准范围内，未影响兔子血红素生物合成过程。
