Harchali A A, Montagne P, Cuillière M L, Bouanani M, Pau B, Duheille J
Immunology Laboratory, Faculty of Medicine, Vandoeuvre-les-Nancy, France.
Clin Chem. 1992 Sep;38(9):1859-64.
To hydrophilic, polyfunctional spherical microparticles of predetermined diameter, produced by copolymerization of acrylic monomers, we covalently bound human thyroglobulin. The thyroglobulin-microsphere conjugate was agglutinated, in the presence of antimouse immunoglobulins antiserum, by four monoclonal antibodies, each recognizing a different antigenic domain on the thyroglobulin molecule. These agglutinations were quantified by measuring with a specially designed nephelometer the light scattered by clusters of the conjugates. Agglutination with the monoclonal antibody recognizing antigenic domain II of the thyroglobulin molecule was specifically inhibited by some human sera that contained antithyroglobulin autoantibodies. This allowed us to develop a microparticle-enhanced nephelometric immunoassay for these autoantibodies with defined epitopic specificity. Using this assay, we detected and quantified antithyroglobulin autoantibodies in serum samples from all eight patients examined with Hashimoto disease and from most (75%) patients with untreated Graves disease.
我们将人甲状腺球蛋白共价结合到通过丙烯酸单体共聚制备的具有预定直径的亲水性、多官能团球形微粒上。在抗小鼠免疫球蛋白抗血清存在的情况下，甲状腺球蛋白 - 微球偶联物被四种单克隆抗体凝集，每种单克隆抗体识别甲状腺球蛋白分子上不同的抗原结构域。通过使用专门设计的散射比浊计测量偶联物簇散射的光来对这些凝集反应进行定量。识别甲状腺球蛋白分子抗原结构域II的单克隆抗体引起的凝集反应被一些含有抗甲状腺球蛋白自身抗体的人血清特异性抑制。这使我们能够开发一种针对这些具有明确表位特异性的自身抗体的微粒增强散射比浊免疫测定法。使用该测定法，我们在所有八名接受检查的桥本氏病患者以及大多数（75%）未经治疗的格雷夫斯病患者的血清样本中检测并定量了抗甲状腺球蛋白自身抗体。
