Misúrová E, Kropácová K
Department of Cellular and Molecular Biology, Faculty of Science, P. J. Safárik University, Kosice, Czechoslovakia.
Neoplasma. 1992;39(1):29-33.
In the blood of mice irradiated with a single total-body dose of 8 Gy gamma rays we examined the concentration of nucleic acids and incorporation of 3H-thymidine and 14C-orotic acid. The concentration of nucleic acids, above all that of DNA in the so-called blood leukocyte mass dropped significantly in the course of the first three days after irradiation. Recovery began in the course of the second week following irradiation. On day 21 after irradiation, DNA concentration was found to be recovered temporarily and RNA concentration to exceed the control values. The increase of incorporation of 3H-thymidine confirmed an increased incidence of DNA synthesizing cells in the blood within days 12-15 after irradiation. Since the increase of incorporation of 3H-thymidine into DNA was not preceded by an increase of incorporation of 14C-orotic acid into RNA, we presume that the DNA synthesizing cells in the blood of irradiated animals do not represent the stimulated lymphocytes but circulating hemopoietic cells. Therefore, the examination of DNA synthesis in blood cells enables to record the course of bone marrow regeneration after irradiation or after application of hemopoiesis depressing substances.
在接受单次全身8 Gy γ射线照射的小鼠血液中，我们检测了核酸浓度以及³H-胸腺嘧啶核苷和¹⁴C-乳清酸的掺入情况。照射后的头三天内，核酸浓度，尤其是所谓血液白细胞群体中的DNA浓度显著下降。照射后第二周开始恢复。照射后第21天，发现DNA浓度暂时恢复，RNA浓度超过对照值。³H-胸腺嘧啶核苷掺入量的增加证实了照射后第12 - 15天血液中DNA合成细胞的发生率增加。由于³H-胸腺嘧啶核苷掺入DNA量的增加之前，¹⁴C-乳清酸掺入RNA量并未增加，我们推测受照射动物血液中的DNA合成细胞并非受刺激的淋巴细胞，而是循环造血细胞。因此，检测血细胞中的DNA合成能够记录照射后或应用造血抑制物质后骨髓再生的过程。
