FINCK H
J Biophys Biochem Cytol. 1960 Feb;7(1):27-30. doi: 10.1083/jcb.7.1.27.
A method of embedding biological specimens in araldite 502 (Ciba) has been developed for materials available in the United States. Araldite-embedded tissues are suitable for electron microscopy, but the cutting qualities of the resin necessitates more than routine attention during microtomy. The rather high viscosity of araldite 502 also seems to be an unnecessary handicap. The less viscous epoxy epon 812 (Shell) produces specimens with improved cutting qualities, and has several features-low shrinkage and absence of specimen damage during cure, minimal compression of sections, relative absence of electron beam-induced section damage, etc.-which recommends it as a routine embedding material. The hardness of the cured resin can be easily adjusted by several methods to suit the materials embedded in it. Several problems and advantages of working with sections of epoxy resins are also discussed.
已开发出一种用阿拉尔代特502（汽巴公司）包埋生物标本的方法，适用于美国现有的材料。用阿拉尔代特包埋的组织适用于电子显微镜检查，但树脂的切片质量要求在切片过程中比常规操作更需注意。阿拉尔代特502相当高的粘度似乎也是一个不必要的障碍。粘度较低的环氧树脂Epon 812（壳牌公司）制作的标本切片质量有所提高，并且具有几个特点——固化过程中收缩率低、标本无损伤、切片压缩最小、电子束引起的切片损伤相对较少等——这些特点使其成为一种常规包埋材料。固化树脂的硬度可以通过几种方法轻松调节，以适应包埋其中的材料。还讨论了使用环氧树脂切片的几个问题和优点。
