Mouelle P, Menu P, Labrude P, Grandgeorge M, Vigneron C
Faculté de Pharmacie, Nancy France.
Biomater Artif Cells Immobilization Biotechnol. 1992;20(2-4):697-701. doi: 10.3109/10731199209119705.
Hemodynamic parameters of six groups of guinea-pig hearts were studied by the working heart technic of Neely. Three groups were respectively perfused with: Krebs-Henseleit, purified native hemoglobin 1gm/dl and hemoglobin conjugated to dextran benzene tetracarboxylate 1 gm/dl. Three other groups were perfused under the same conditions except that after 30 mn of perfusion, 10 mn of total ischemia were produced followed by 30 mn of reperfusion with the previous solutions. All solutions contained 10 g/l of BSA. Hearts perfused with Hb solutions without ischemia or after ischemia show better parameters than with Krebs-Henseleit. These observations suggest that contrary to previously published results, purified Hb and more, dextran-BTC-Hb appear to be perfusable and are less deleterious for heart than saline without hemoglobin.
采用尼利的工作心脏技术研究了六组豚鼠心脏的血流动力学参数。三组分别用以下溶液灌注：克雷布斯 - 亨泽莱特溶液、每分升含1克纯化天然血红蛋白的溶液以及每分升含与苯四羧酸葡聚糖共轭血红蛋白1克的溶液。另外三组在相同条件下灌注，但在灌注30分钟后造成10分钟的完全缺血，然后用先前的溶液再灌注30分钟。所有溶液均含有每升10克的牛血清白蛋白。未经历缺血或缺血后用血红蛋白溶液灌注的心脏，其各项参数比用克雷布斯 - 亨泽莱特溶液灌注时更好。这些观察结果表明，与先前发表的结果相反，纯化血红蛋白，尤其是葡聚糖 - BTC - 血红蛋白似乎是可灌注的，并且对心脏的危害比不含血红蛋白的生理盐水更小。
