Faivre B, Labaeye V, Menu P, Labrude P, Vigneron C
Laboratoire d'Hématologie-Physiologie, Faculté de Pharmacie, Nancy, France.
Artif Cells Blood Substit Immobil Biotechnol. 1995;23(4):495-504. doi: 10.3109/10731199509117965.
With the aim of assessing of dextran-benzene-tetracarboxylate hemoglobin as an oxygen carrier, we studied histological changes in the intestinal loop in anesthetized guinea pig. The intestinal tissue being very sensitive to hypoxia, an innervated loop was vascularly perfused with open-flow during one hour at zero hematocrit. To estimate the capacity of hemoglobin solution to oxygenate this tissue, we observed the mechanical and histological changes in the organ and the arterio-venous difference in PO2, oxyhemoglobin, deoxyhemoglobin and we compared them with human albumin, Tyrode and non-modified hemoglobin. The PO2 arteriovenous differences were 51.9 +/- 7.1 torr (m +/- SEM) for Tyrode, 40.2 +/- 6.4 torr for albumin solution, 113.7 +/- 6.5 torr for non-modified hemoglobin and 132.7 +/- 6.8 torr for dex-BTC-Hb. Compared to albumin and Tyrode solutions, hemoglobin solutions transferred more oxygen to tissues. The desaturation of dex-BTC-Hb was significantly superior (p < 0.05) to the one non-modified hemoglobin. With Hb solutions, this desaturation increased with time and it depended on the perfusion flow. The structure of jejunal villi when perfused with a hemoglobin solution, remained almost normal and the loop was still active. Nevertheless, non-modified hemoglobin leaked from the vessels to the lumen and caused edema and a rupture of overlapping epithelium at the tip of the villi. With dex-BTC-Hb, such histological modifications were less significant. With albumin and Tyrode, all villi were totally necrosed and the loop was completely inert. We have demonstrated that dextran-benzene-tetracarboxylate hemoglobin had the ability to maintain the tissue alive thanks to its good capacity to release oxygen and its satisfactory vascular persistence. Dex-BTC-Hb solution can answer to needs of tissue.
为了评估葡聚糖 - 苯四羧酸盐血红蛋白作为氧载体的性能，我们研究了麻醉豚鼠肠袢的组织学变化。肠组织对缺氧非常敏感，在零血细胞比容下，对一个有神经支配的肠袢进行一小时的开放流血管灌注。为了评估血红蛋白溶液对该组织进行氧合的能力，我们观察了器官的机械和组织学变化以及动脉 - 静脉间的氧分压、氧合血红蛋白、脱氧血红蛋白差异，并将它们与人体白蛋白、台氏液和未修饰的血红蛋白进行比较。台氏液的动脉 - 静脉氧分压差异为51.9±7.1托（平均值±标准误），白蛋白溶液为40.2±6.4托，未修饰的血红蛋白为113.7±6.5托，葡聚糖 - BTC - 血红蛋白为132.7±6.8托。与白蛋白和台氏液相比，血红蛋白溶液向组织输送了更多的氧气。葡聚糖 - BTC - 血红蛋白的去饱和明显优于未修饰的血红蛋白（p < 0.05）。使用血红蛋白溶液时，这种去饱和随时间增加，并且取决于灌注流量。用血红蛋白溶液灌注时，空肠绒毛的结构几乎保持正常，肠袢仍有活性。然而，未修饰的血红蛋白从血管渗漏到管腔，导致绒毛尖端重叠上皮的水肿和破裂。使用葡聚糖 - BTC - 血红蛋白时，这种组织学改变不太明显。使用白蛋白和台氏液时，所有绒毛完全坏死，肠袢完全无活性。我们已经证明，葡聚糖 - 苯四羧酸盐血红蛋白由于其良好的氧释放能力和令人满意的血管持久性，有能力维持组织存活。葡聚糖 - BTC - 血红蛋白溶液可以满足组织的需求。
