Stromer M, Hasselbach W
Z Naturforsch C Biosci. 1976 Nov-Dec;31(11-12):703-7.
Fragmented sarcoplasmic reticulum (FSR) vesicles from rabbit muscle were suspended in 1.5-5% glycerol solutions and were pelleted onto aluminum foil disks in a modified centrifuge tube. Examination of these pellets in the electron microscope after drying for 2--2.5, 4--5.5, and 21 hours revealed a progression of changes. First, distances between individual, round vesicles decreases. Next, somewhat flattened vesicles establish limited areas of contact with adjacent vesicles. Finally, vesicle fusion occurs and extended areas of double bilayers are formed. A water loss-time interaction appears to be needed for the fusion process. A Hg-phenyl azoferritin compound was used as a marker to identify intra- and extra-vesicular space in the fused samples. Quantitative measurements of birefringence during imbibition of pellet slices in a graded series (eta-1.41-1.43) is reached. The plateau seen in this part of the curve is again followed by steadily increasing birefringence at higher glycerol concentrations. This interruption in the birefringence curve is presumably due to a matching of the refractive indices of the glycerol solution and a lipid component in the membranes.
将来自兔肌肉的破碎肌质网（FSR）囊泡悬浮于1.5% - 5%的甘油溶液中，并在改良的离心管中沉淀到铝箔圆盘上。在干燥2 - 2.5小时、4 - 5.5小时和21小时后，用电子显微镜检查这些沉淀，发现了一系列变化。首先，单个圆形囊泡之间的距离减小。接着，有些扁平的囊泡与相邻囊泡建立起有限的接触区域。最后，囊泡发生融合，形成了双层膜的扩展区域。融合过程似乎需要水分流失与时间的相互作用。使用汞 - 苯基偶氮铁蛋白化合物作为标记物来识别融合样品中的囊泡内和囊泡外空间。在梯度系列（η = 1.41 - 1.43）的甘油溶液中浸泡沉淀切片时，对双折射进行了定量测量。在该曲线的这一部分出现的平台期之后，在更高甘油浓度下双折射又稳步增加。双折射曲线中的这种中断大概是由于甘油溶液和膜中脂质成分的折射率匹配所致。
