Stromer M, The R, Hasselbach W
Z Naturforsch C Biosci. 1976 Nov-Dec;31(11-12):708-11. doi: 10.1515/znc-1976-11-1215.
Fragmented sarcoplasmic reticulum (FSR) vesicles from rabbit skeletal muscle were suspended in 5-10% ethylene glycol (EG) or in 5, 10, or 15% dimethylsulfoxide (DMSO) and were pelleted onto flat aluminum foil disks. No vesicle fusion o-curs with either 5 or 10% EG treatment and 2-21/2 hours drying. After 4-5 hours drying, 5% EG-treated vesicles have established more areas of close contact and individual vesicles have begun to flatten when compared with the 10% EG vesicles remained round as in the 2-21/2 hour samples of all treatments. Overnight drying also causes extended sheets of bilayer pairs to form in the 5% DMSO-treated samples but, with 10 and 25% treated vesicles, destroys the double bilayers and only occasional dense regions of membrane whorls remain. Both EG and DMSO promote morerapid fusion of FSR vesicles than does glycerol but overnight drying after treatment with 10 or 25% DMSO DESTROYS THE FUSED MEMBRANE.
来自兔骨骼肌的肌浆网碎片（FSR）囊泡悬浮于5 - 10%的乙二醇（EG）或5%、10%或15%的二甲基亚砜（DMSO）中，然后沉淀到扁平的铝箔圆盘上。经5%或10%的EG处理并干燥2 - 2.5小时后，未发生囊泡融合。干燥4 - 5小时后，与10% EG处理的囊泡相比，5% EG处理的囊泡形成了更多紧密接触区域，且单个囊泡开始变平，而10% EG处理的囊泡仍保持圆形，如同所有处理2 - 2.5小时时的样本。过夜干燥还会使5% DMSO处理的样本中形成双层对的延伸片层，但对于10%和25%处理的囊泡，会破坏双层结构，仅残留偶尔的膜涡旋致密区域。与甘油相比，EG和DMSO都能促进FSR囊泡更快融合，但用10%或25% DMSO处理后过夜干燥会破坏融合膜。
