在克利夫顿伞藻中鉴定出两种生物钟蛋白，并构建了克利夫顿伞藻和地中海伞藻的cDNA文库。

Identification of two clock proteins in Acetabularia cliftonii and construction of cDNA libraries from Acetabularia cliftonii and Acetabularia mediterranea.

Yang X P, de Groot E J

Max-Planck-Institut für Zellbiologie, Abteilung Schweiger, Ladenburg, Fed. Rep. Germany.

Int J Biochem. 1992 Jul;24(7):1141-50. doi: 10.1016/0020-711x(92)90385-e.

Abstract

Two clock proteins were identified in A. cliftonii. The first has a molecular weight (mol. wt) of 200 kDa (P200) and its synthesis shows a 24 hr periodicity. The second has mol. wt of 130 kDa (P130) and shows a semicircadian rhythm with a periodicity of about 12 hr. 2. cDNA libraries from A. cliftonii and A. mediterranea were prepared by cloning cDNA in lambda gt10 and lambda gt11, respectively. 3. One clone each of the two libraries hybridized with the human beta-actin pseudogene. One clone of the A. mediterranea and 4 clones of the A. cliftonii libraries hybridized to Chlamydomonas heat-shock gene.

摘要

在克利夫顿衣藻中鉴定出两种生物钟蛋白。第一种分子量为200 kDa（P200），其合成表现出24小时的周期性。第二种分子量为130 kDa（P130），表现出约12小时周期性的半昼夜节律。2. 分别通过将cDNA克隆到λgt10和λgt11中，制备了来自克利夫顿衣藻和地中海衣藻的cDNA文库。3. 两个文库各有一个克隆与人类β-肌动蛋白假基因杂交。地中海衣藻文库的一个克隆和克利夫顿衣藻文库的4个克隆与衣藻热休克基因杂交。