编码小麦贮藏蛋白的cDNA克隆的特性分析。
The characterization of cDNA clones coding for wheat storage proteins.
作者信息
Bartels D, Thompson R D
出版信息
Nucleic Acids Res. 1983 May 25;11(10):2961-77. doi: 10.1093/nar/11.10.2961.
Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley storage protein, B-hordein. The sequence includes a tandem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.
从小麦品种中国春发育中的胚乳中分离出的聚腺苷酸加尾RNA（Poly(A)+ RNA）已被用作构建cDNA文库的模板。在该文库中，通过对杂交筛选的mRNA进行体外翻译，鉴定出了编码α/β醇溶蛋白相关序列和γ-醇溶蛋白相关序列的克隆。已确定了其中一个这样的克隆的DNA序列，它与编码大麦贮藏蛋白B-大麦醇溶蛋白的克隆的序列具有同源性。该序列包含一个串联DNA重复序列，文中结合醇溶蛋白多样性的产生对其进行了讨论。
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