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编码小麦贮藏蛋白的cDNA克隆的特性分析。

The characterization of cDNA clones coding for wheat storage proteins.

作者信息

Bartels D, Thompson R D

出版信息

Nucleic Acids Res. 1983 May 25;11(10):2961-77. doi: 10.1093/nar/11.10.2961.

DOI:10.1093/nar/11.10.2961
PMID:6190127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325941/
Abstract

Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley storage protein, B-hordein. The sequence includes a tandem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.

摘要

从小麦品种中国春发育中的胚乳中分离出的聚腺苷酸加尾RNA（Poly(A)+ RNA）已被用作构建cDNA文库的模板。在该文库中，通过对杂交筛选的mRNA进行体外翻译，鉴定出了编码α/β醇溶蛋白相关序列和γ-醇溶蛋白相关序列的克隆。已确定了其中一个这样的克隆的DNA序列，它与编码大麦贮藏蛋白B-大麦醇溶蛋白的克隆的序列具有同源性。该序列包含一个串联DNA重复序列，文中结合醇溶蛋白多样性的产生对其进行了讨论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/83379ee05094/nar00355-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/b4f329710b78/nar00355-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/f061d0f9b510/nar00355-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/83379ee05094/nar00355-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/b4f329710b78/nar00355-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/f061d0f9b510/nar00355-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f7ef/325941/83379ee05094/nar00355-0022-a.jpg

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引用本文的文献

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本文引用的文献

1
Wheat Storage Proteins : ISOLATION AND CHARACTERIZATION OF THE GLIADIN MESSENGER RNAs.小麦贮藏蛋白：麦醇溶蛋白信使核糖核酸的分离与特性分析
Plant Physiol. 1982 Apr;69(4):834-9. doi: 10.1104/pp.69.4.834.
2
Cloning and sequence analysis reveal structural variation among related zein genes in maize.克隆与序列分析揭示了玉米中相关醇溶蛋白基因间的结构变异。
Cell. 1982 Jul;29(3):1015-26. doi: 10.1016/0092-8674(82)90465-2.
3
A structural model for maize zein proteins.玉米醇溶蛋白的结构模型。
番茄多聚半乳糖醛酸酶及其它成熟相关蛋白 cDNA 克隆的分离和特性分析。
Plant Mol Biol. 1985 May;5(3):137-47. doi: 10.1007/BF00015677.
4
cDNA and protein sequence of a major pea seed albumin (PA 2 : Mr≈26 000).豌豆种子主要清蛋白（PA 2 ：Mr≈26000）的 cDNA 和蛋白质序列。
Plant Mol Biol. 1987 Jan;8(1):37-45. doi: 10.1007/BF00016432.
5
Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones.利用 cDNA 克隆对小麦高分子量麦谷蛋白亚基的多基因家族进行特征分析。
Theor Appl Genet. 1983 Nov;67(1):87-96. doi: 10.1007/BF00303930.
6
Structural homology of endosperm high molecular weight glutenin subunits of common wheat (Triticum aestivum L.).普通小麦（Triticum aestivum L.）胚乳高分子量麦谷蛋白亚基的结构同源性。
Theor Appl Genet. 1985 Sep;70(6):634-42. doi: 10.1007/BF00252289.
7
Molecular analysis of γ-gliadin gene families at the complex Gli-1 locus of bread wheat (T. aestivum L.).小麦 Gli-1 位点 γ-醇溶蛋白基因家族的分子分析。
Theor Appl Genet. 1986 Sep;72(6):845-53. doi: 10.1007/BF00266556.
8
RFLP analysis and linkage mapping in Solanum tuberosum.马铃薯的 RFLP 分析和连锁图谱
Theor Appl Genet. 1989 Jul;78(1):65-75. doi: 10.1007/BF00299755.
9
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Planta. 1988 Oct;175(4):485-92. doi: 10.1007/BF00393069.
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Theor Appl Genet. 1991 May;81(5):653-60. doi: 10.1007/BF00226733.
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4
Sequence analysis and comparison of cDNAs of the zein multigene family .玉米醇溶蛋白多基因家族cDNA的序列分析与比较
EMBO J. 1982;1(11):1329-35. doi: 10.1002/j.1460-2075.1982.tb01318.x.
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6
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