CD1a、CD1b和CD1c抗原的表位在人皮肤的朗格汉斯细胞、真皮树突状细胞、角质形成细胞和基底膜带中呈差异定位。
Epitopes for CD1a, CD1b, and CD1c antigens are differentially mapped on Langerhans cells, dermal dendritic cells, keratinocytes, and basement membrane zone in human skin.
作者信息
Furue M, Nindl M, Kawabe K, Nakamura K, Ishibashi Y, Sagawa K
机构信息
Department of Dermatology, University of Tokyo, Japan.
出版信息
J Am Acad Dermatol. 1992 Sep;27(3):419-26. doi: 10.1016/0190-9622(92)70211-w.
BACKGROUND
CD1 antigens are classified serologically into at least three groups, CD1a, CD1b, and CD1c, and many kinds of monoclonal antibodies are available for each subgroup of CD1 antigens. CD1a, CD1b, and CD1c antigens have been shown to be selectively and differentially expressed on epidermal Langerhans cells and dermal dendritic cells in normal human skin.
OBJECTIVE
The objective was to further delineate the localization of epitopes of CD1 antigens in human skin.
METHODS
We examined the immunoreactivity of 14 different CD1 antibodies (seven CD1a, five CD1b, and two CD1c antibodies) with the immunoperoxidase technique. We also studied the reactivity of NU-T2 (CD1b) antibody by immunogold electron microscopy.
RESULTS
The epitopes for CD1a, CD1b, and CD1c antigens were differentially mapped on epidermal Langerhans cells, dermal dendritic cells, keratinocytes, the luminal portion of eccrine gland ducts, and the basement membrane zone in human skin.
CONCLUSION
These CD1 antibodies may be useful to analyze the phenotypic alteration of immune and nonimmune cells in various skin diseases.
背景
CD1抗原在血清学上至少分为三组，即CD1a、CD1b和CD1c，并且针对CD1抗原的每个亚组都有多种单克隆抗体。已证实在正常人类皮肤中，CD1a、CD1b和CD1c抗原在表皮朗格汉斯细胞和真皮树突状细胞上选择性且差异性表达。
目的
进一步明确CD1抗原表位在人类皮肤中的定位。
方法
我们采用免疫过氧化物酶技术检测了14种不同的CD1抗体（7种CD1a抗体、5种CD1b抗体和2种CD1c抗体）的免疫反应性。我们还通过免疫金电子显微镜研究了NU-T2（CD1b）抗体的反应性。
结果
CD1a、CD1b和CD1c抗原的表位在人类皮肤的表皮朗格汉斯细胞、真皮树突状细胞、角质形成细胞、小汗腺导管管腔部分和基底膜带呈差异性定位。
结论
这些CD1抗体可能有助于分析各种皮肤疾病中免疫细胞和非免疫细胞的表型改变。
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