Akagi T
Acta Med Okayama. 1976 Dec;30(6):385-95.
The fetal antigen(s) of C3H/BfB/Ki mouse tumor cells induced by adenovirus type 12 was examined by transplantation resistance, local adoptive transfer and in vitro cell-mediated microcytotoxicity tests. Mice immunized with 8 to 9-day gestation fetal cells showed weak resistance to the challenge of small numbers of tumor cells, but immunization with 13 to 14-day gestation fetal cells did not confer transplantation immunity. Local adoptive transfer of multiparous pregnant mouse spleen cells mixed with tumor cells showed a small inhibition of tumor growth in all cases, but primiparous pregnant mouse spleen cells showed a protective effect in only one of three samples. In the cell-mediated microcytotoxicity test, spleen cells taken from the fetal tissue-immunized or multiparous pregnant mice showed a cytotoxic effect to MT-2 cells (adenovirus type 12 tumor cell line) and mouse fetal cells, but not to newborn mouse kidney cells. These results strongly suggest the presence of phase-specific tumor-associated fetal antigen(s) which can evoke transplantation immunity.
通过移植抗性、局部过继转移和体外细胞介导的微细胞毒性试验，对12型腺病毒诱导的C3H/BfB/Ki小鼠肿瘤细胞的胎儿抗原进行了检测。用妊娠8至9天的胎儿细胞免疫的小鼠，对少量肿瘤细胞的攻击表现出较弱的抗性，但用妊娠13至14天的胎儿细胞免疫则未赋予移植免疫力。将多胎妊娠小鼠的脾细胞与肿瘤细胞混合进行局部过继转移，在所有情况下均显示出对肿瘤生长的轻微抑制，但初胎妊娠小鼠的脾细胞仅在三个样本中的一个中表现出保护作用。在细胞介导的微细胞毒性试验中，取自经胎儿组织免疫的或多胎妊娠小鼠的脾细胞对MT-2细胞（12型腺病毒肿瘤细胞系）和小鼠胎儿细胞有细胞毒性作用，但对新生小鼠肾细胞无细胞毒性作用。这些结果强烈提示存在能引发移植免疫的阶段特异性肿瘤相关胎儿抗原。
