评估“长链反应”作为检测人血清中A群链球菌型特异性抗体的一种方法。
Evaluation of the "long chain reaction" as a means for detecting type-specific antibody to group a streptococci in human sera.
作者信息
STOLLERMAN G H, SIEGEL A C, JOHNSON E E
出版信息
J Exp Med. 1959 Dec 1;110(6):887-97. doi: 10.1084/jem.110.6.887.
Abstract
Certain strains of Group A streptococci showed striking increase in chain length when grown in liquid media to which was added human sera that contained antibody to M protein of homologous type. This "long chain reaction" was shown to be a highly specific and sensitive biological test for human type-specific antibody and correlated closely with the classical bactericidal test. Patients infected with Type 12 or Type 3 Group A streptococci showed the appearance of anti-M antibody in their sera by both methods at similar intervals during convalescence. Of 217 sera studied in these patients the two tests showed agreement in all but 11 specimens. Of 99 patients who were bled serially following Type 12 or Type 3 infections, and whose sera were tested by both methods, there was close agreement, the bactericidal test being only slightly more sensitive. The advantages and limitations of this new biological test for human type-specific immunity are discussed.
摘要
当A组链球菌的某些菌株在添加了含有同源型M蛋白抗体的人血清的液体培养基中生长时，其链长显著增加。这种“长链反应”被证明是一种针对人型特异性抗体的高度特异性和灵敏的生物学检测方法，并且与经典的杀菌试验密切相关。感染12型或3型A组链球菌的患者在康复期间，通过这两种方法在相似的时间间隔内血清中均出现了抗M抗体。在这些患者中研究的217份血清中，除11份标本外，两种检测方法结果一致。在99例12型或3型感染后连续采血且血清用两种方法检测的患者中，结果高度一致，杀菌试验仅稍灵敏一些。本文讨论了这种用于检测人型特异性免疫的新生物学检测方法的优点和局限性。
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J Exp Med. 1959 Dec 1;110(6):887-97. doi: 10.1084/jem.110.6.887.
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