Yuan H, Zhang Z
Department of Biology, Nankai University, Tianjin, China.
Mutat Res. 1992 Oct;272(2):125-31.
A study of some factors affecting sister-chromatid differentiation (SCD) and sister-chromatid exchanges (SCE) in Hordeum vulgare is reported. After we studied the influence of 5-fluorodeoxyuridine (FdU) and growth temperature on SCE in barley cells, and the effect of FdU, growth temperature, the growth time of plant cells in 5-bromo-2-deoxyuridine (BrdU) solution on SCD, we found an experimental condition under which the frequency of SCE is lower, but the percentage of SCD is higher. Our data show that ascorbic acid, mitomycin C, adriamycin, and maleic hydrazide induce SCEs in cells of Hordeum vulgare by means of free radicals. This can be shown from the two observations: (1) sulfhydryl compounds such as cysteine and glutathione can completely or partially inhibit the SCEs induced by ascorbic acid, mitomycin C, adriamycin and maleic hydrazide; (2) the amounts of free radicals in root tips correlate with the frequencies of SCE in root tip cells.
本文报道了一项关于影响大麦姐妹染色单体分化（SCD）和姐妹染色单体交换（SCE）的某些因素的研究。在我们研究了5-氟脱氧尿苷（FdU）和生长温度对大麦细胞中SCE的影响，以及FdU、生长温度、植物细胞在5-溴-2-脱氧尿苷（BrdU）溶液中的生长时间对SCD的影响之后，我们发现了一种实验条件，在此条件下SCE的频率较低，但SCD的百分比更高。我们的数据表明，抗坏血酸、丝裂霉素C、阿霉素和顺丁烯二酸酰肼通过自由基诱导大麦细胞中的SCE。这可以从以下两个观察结果中看出：（1）半胱氨酸和谷胱甘肽等巯基化合物可以完全或部分抑制抗坏血酸、丝裂霉素C、阿霉素和顺丁烯二酸酰肼诱导的SCE；（2）根尖中的自由基数量与根尖细胞中SCE的频率相关。
