马来酰肼对洋葱根尖染色体姐妹染色单体交换产生影响的因素。
Factors affecting the production of SCEs by maleic hydrazide in root-tip chromosomes of Allium cepa.
作者信息
Cortés F, Escalza P, Mateos S, Díaz-Recasens M
机构信息
Departamento de Citología e Histología Vegetal y Animal, Facultad de Biología, Sevilla, Spain.
出版信息
Mutat Res. 1987 Oct;192(2):125-30. doi: 10.1016/0165-7992(87)90108-4.
We have investigated the influence of pH on the induction of chromatid-type aberrations and sister-chromatid exchanges (SCEs) by maleic hydrazide (MH) in root-tip cells of Allium cepa. For both cytogenetic endpoints, the lower the pH of the treatment solution, the higher were the frequencies of chromosome alterations detected at metaphase. We have further studied the persistence of lesions giving rise to SCEs during successive cell cycles, as well as the influence of BrdU concentration in the post-treatment medium on the yield of MH-induced SCEs. Our results suggest that the cytogenetic action of MH in many respects resembles that of bifunctional alkylating agents.
我们研究了pH值对马来酰肼（MH）诱导洋葱根尖细胞染色单体型畸变和姐妹染色单体交换（SCEs）的影响。对于这两个细胞遗传学终点，处理溶液的pH值越低，在中期检测到的染色体改变频率就越高。我们进一步研究了在连续细胞周期中产生SCEs的损伤的持续性，以及处理后培养基中BrdU浓度对MH诱导的SCEs产量的影响。我们的结果表明，MH在许多方面的细胞遗传学作用类似于双功能烷化剂。
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