氨基酸对姐妹染色单体交换的影响。

Effects of amino acids on sister-chromatid exchanges.

作者信息

Zhang Z, Yang J

机构信息

Department of Biology, Nankai University, Tianjin, China.

出版信息

Mutat Res. 1992 Oct;280(4):279-83. doi: 10.1016/0165-1218(92)90058-8.

DOI:10.1016/0165-1218(92)90058-8

PMID:1382229

Abstract

The effects of 10 amino acids on sister-chromatid exchange (SCE) frequency in human peripheral blood lymphocytes (PBL) and six amino acids on the SCE frequency in root tip cells of Hordeum vulgare were studied. Alanine (Ala), glycine (Gly), phenylalanine (Phe), valine (Val), histidine (His) and serine (Ser) induced a significant increase in SCE in PBL but threonine (Thr), isoleucine (Ile), lysine (Lys) and arginine (Arg) did not. Ala, Gly, Thr, Ile and Val induced a significant increase in SCE in root tip cells of Hordeum vulgare but Lys did not. The effect of Lys and bromodeoxyuridine (BrdU) on SCE levels in PBL and the interaction between them were also studied. The results show that Lys can inhibit the SCE induced by BrdU.

摘要

研究了10种氨基酸对人外周血淋巴细胞（PBL）姐妹染色单体交换（SCE）频率的影响，以及6种氨基酸对大麦根尖细胞SCE频率的影响。丙氨酸（Ala）、甘氨酸（Gly）、苯丙氨酸（Phe）、缬氨酸（Val）、组氨酸（His）和丝氨酸（Ser）可显著增加PBL中的SCE，但苏氨酸（Thr）、异亮氨酸（Ile）、赖氨酸（Lys）和精氨酸（Arg）则不能。Ala、Gly、Thr、Ile和Val可显著增加大麦根尖细胞中的SCE，但Lys则不能。还研究了赖氨酸和溴脱氧尿苷（BrdU）对PBL中SCE水平的影响及其相互作用。结果表明，Lys可抑制BrdU诱导的SCE。