Chen Y, Laburthe M, Amiranoff B
INSERM U 239, Paris, France.
Peptides. 1992 Mar-Apr;13(2):339-41. doi: 10.1016/0196-9781(92)90118-m.
The ubiquitous neuropeptide, galanin, strongly inhibits adenylate cyclase in rat brain membranes. While basal enzyme activity was not altered, galanin from 10(-11) M to 5 x 10(-7) M decreased forskolin- and VIP-stimulated adenylate cyclase with a half-maximal effect being elicited by 0.7 nM neuropeptide and a maximal 80% inhibition of the enzyme activity. The galanin fragments (2-29) and (1-15) dose-dependently inhibited the forskolin-stimulated adenylate cyclase, while the fragments (3-29) and (10-29) were found inactive. These results indicate that the regulatory action of galanin in the central nervous system involves the coupling of galanin receptors to the inhibition of the adenylate cyclase system.
普遍存在的神经肽甘丙肽能强烈抑制大鼠脑膜中的腺苷酸环化酶。虽然基础酶活性未改变，但浓度在10^(-11) M至5×10^(-7) M之间的甘丙肽可降低福斯高林和血管活性肠肽刺激的腺苷酸环化酶活性，0.7 nM神经肽可产生半数最大效应，且对该酶活性的最大抑制率为80%。甘丙肽片段（2 - 29）和（1 - 15）呈剂量依赖性地抑制福斯高林刺激的腺苷酸环化酶，而片段（3 - 29）和（10 - 29）则无活性。这些结果表明，甘丙肽在中枢神经系统中的调节作用涉及甘丙肽受体与腺苷酸环化酶系统抑制的偶联。
