Sainte-Laudy J, Vallon C, Guerin J C
Centre de biologie, Lyon.
Allerg Immunol (Paris). 1992 Oct;24(8):298, 301-5.
Within a context of a familial case of severe respiratory signs, with intolerance of sulfites and aspirin, we decided to study the clinical reliability of "in vitro" activation of human basophils in the presence of sulfites. Positivity of these tests for the three patients in the study (60, 73, and 66%) is confirmed by passive sensitization of donor basophils, which was destroyed when the serum was heated. This argument in favour of IgE dependence was strengthened by observation of an "in vitro" desensitization by an anti-IgE and not by an anti-IgG. These "in vitro" data are in agreement with the clinical history, daily routine of the patient, skin tests and where done, oral provocation tests.
在一个有严重呼吸道症状、对亚硫酸盐和阿司匹林不耐受的家族病例背景下，我们决定研究在亚硫酸盐存在时人嗜碱性粒细胞“体外”激活的临床可靠性。研究中的三名患者这些测试的阳性率（分别为60%、73%和66%）通过供体嗜碱性粒细胞的被动致敏得到证实，当血清加热时致敏作用被破坏。通过观察抗IgE而非抗IgG的“体外”脱敏作用，支持IgE依赖性的这一论点得到了加强。这些“体外”数据与患者的临床病史、日常情况、皮肤试验以及（如有进行）口服激发试验结果一致。
