三环类镇静剂激活的蜂毒肽通道的23Na和1H核磁共振研究。
23Na and 1H NMR studies on melittin channels activated by tricyclic tranquilizers.
作者信息
Tanaka H, Matsunaga K, Kawazura H
机构信息
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
出版信息
Biophys J. 1992 Aug;63(2):569-72. doi: 10.1016/S0006-3495(92)81629-6.
Abstract
A dynamic 23Na nuclear magnetic resonance (NMR) technique was applied to the exchange system of Na+ ions present inside and outside large unilamellar vesicles at an equivalent concentration. Addition of melittin to phosphatidylcholine vesicles did not induce any detectable Na+ transport across the membrane but subsequent addition of a trace of chlorpromazine or imipramine did induce Na+ transport. Because the formation of a drug-melittin adduct in a solution was detected by 1H NMR, the activation of melittin channels was assumed to originate from the direct interaction of the drug and melittin.
摘要
一种动态23Na核磁共振（NMR）技术被应用于等浓度存在于大单层囊泡内外的Na+离子交换系统。向磷脂酰胆碱囊泡中添加蜂毒素不会诱导任何可检测到的Na+跨膜转运，但随后添加微量的氯丙嗪或丙咪嗪确实会诱导Na+转运。由于通过1H NMR检测到溶液中药物 - 蜂毒素加合物的形成，因此推测蜂毒素通道的激活源于药物与蜂毒素的直接相互作用。
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本文引用的文献
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Activation and inactivation of melittin channels.蜂毒肽通道的激活与失活。
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Interaction of alytic polypeptide, melittin, with lipid membrane systems.溶血多肽蜂毒肽与脂质膜系统的相互作用。 （注：原文中“alytic”可能有误，推测应为“hemolytic”，即溶血的）
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[Sequence analysis of melittin from tryptic and peptic degradation products].[胰蛋白酶和胃蛋白酶降解产物中蜂毒肽的序列分析]
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The membrane actions of anesthetics and tranquilizers.麻醉剂和镇静剂的膜作用。
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9
Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions.作为双层偶联物的生物膜。药物与红细胞相互作用的分子机制。
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4457-61. doi: 10.1073/pnas.71.11.4457.
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The monensin-mediated transport of sodium ions through phospholipid bilayers studied by 23Na-NMR spectroscopy.通过23Na-NMR光谱研究莫能菌素介导的钠离子通过磷脂双层的转运。
Biochim Biophys Acta. 1985 Jul 25;817(2):313-7. doi: 10.1016/0005-2736(85)90033-1.
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