在平衡条件下短杆菌肽诱导离子通过膜运输的23Na核磁共振研究。
23Na-nuclear magnetic resonance investigation of gramicidin-induced ion transport through membranes under equilibrium conditions.
作者信息
Buster D C, Hinton J F, Millett F S, Shungu D C
机构信息
Department of Chemistry/Biochemistry, University of Arkansas, Fayetteville 72701.
出版信息
Biophys J. 1988 Feb;53(2):145-52. doi: 10.1016/S0006-3495(88)83076-5.
Abstract
A technique for investigating the gramicidin-facilitated transport of Na+ ions across lipid bilayers of large unilamellar vesicles under the condition of ionic equilibrium has been developed using a combination of heat incubation of the gramicidin with the vesicles and 23Na-nuclear magnetic resonance (NMR) spectroscopy. Isolation of the two 23Na-NMR signals from the intra- and extravesicular Na+ with the shift reagent, dysprosium (III) tripolyphosphate, allows the equilibrium flux of Na+ through the gramicidin channels to be detected and treated as a two-site exchange process. This study indicates that the transport of Na+ through gramicidin channels is second order with respect to the gramicidin concentration.
摘要
已开发出一种技术，通过将短杆菌肽与大单层囊泡进行热孵育并结合23Na核磁共振（NMR）光谱，来研究在离子平衡条件下短杆菌肽促进Na+离子跨大单层囊泡脂质双层的转运。使用位移试剂三聚磷酸镝从囊泡内和囊泡外的Na+中分离出两个23Na-NMR信号，从而能够检测通过短杆菌肽通道的Na+平衡通量，并将其视为双位点交换过程。这项研究表明，Na+通过短杆菌肽通道的转运相对于短杆菌肽浓度是二级反应。
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本文引用的文献
1
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Biophys J. 1982 Jun;38(3):323-6. doi: 10.1016/S0006-3495(82)84566-9.
2
39K, 23Na, and 31P NMR studies of ion transport in Saccharomyces cerevisiae.酿酒酵母中离子转运的39K、23Na和31P核磁共振研究。
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5185-9. doi: 10.1073/pnas.80.17.5185.
3
Preparation of unilamellar liposomes of intermediate size (0.1-0.2 mumol) by a combination of reverse phase evaporation and extrusion through polycarbonate membranes.通过反相蒸发和经聚碳酸酯膜挤压相结合的方法制备中等大小（0.1 - 0.2 微摩尔）的单层脂质体。
Biochim Biophys Acta. 1980 Oct 2;601(3):559-71. doi: 10.1016/0005-2736(80)90558-1.
4
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