Komuro Y, Ishihara K, Ohara S, Saigenji K, Hotta K
Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan.
Gastroenterol Jpn. 1992 Oct;27(5):597-603. doi: 10.1007/BF02774973.
The effects of tetragastrin on mucus glycoprotein (mucin) metabolism and mucosal protection in rat gastric mucosa were investigated. Rats were administered with various doses of tetragastrin (12, 120, or 400 micrograms/kg body weight; s.c.), followed by 50% ethanol-induced gastric injury. Tetragastrin caused a significant increase in mucin content in the corpus mucosa and prevented 50% ethanol-induced gastric mucosal damage in a dose-dependent manner. For assessment of the effects of tetragastrin on the metabolism of gastric mucin in detail, changes in mucin distribution in the three different layers of rat gastric mucosa were examined one hour after single administration of tetragastrin. A significant increase in the mucin content was noted in the mucus gel and surface mucosal layer. Mucin content in the deep mucosa corresponding mainly to the mucus neck cell mucin underwent virtually no change by this treatment. An increase in mucin in the mucus gel and surface mucosa would thus appear due to the administration of tetragastrin and may possibly be related to the protective action of the gastric mucosa against injury. The data demonstrate a possibility that gastrin may have potential for enhancing gastric mucosal protection associated with mucus secretion and/or mucus synthesis on the surface mucosa of rat gastric mucosa.
研究了四肽胃泌素对大鼠胃黏膜黏液糖蛋白（黏蛋白）代谢及黏膜保护的作用。给大鼠皮下注射不同剂量的四肽胃泌素（12、120或400微克/千克体重），随后给予50%乙醇诱导胃损伤。四肽胃泌素可使胃体黏膜中黏蛋白含量显著增加，并以剂量依赖方式预防50%乙醇诱导的胃黏膜损伤。为详细评估四肽胃泌素对胃黏蛋白代谢的影响，在单次给予四肽胃泌素1小时后，检测大鼠胃黏膜三层不同层中黏蛋白分布的变化。在黏液凝胶层和表面黏膜层中观察到黏蛋白含量显著增加。主要对应于黏液颈细胞黏蛋白的深层黏膜中的黏蛋白含量在该处理后几乎没有变化。因此，给予四肽胃泌素后黏液凝胶层和表面黏膜中的黏蛋白增加，这可能与胃黏膜对损伤的保护作用有关。数据表明胃泌素可能具有增强大鼠胃黏膜表面与黏液分泌和/或黏液合成相关的胃黏膜保护的潜力。
