Azuumi Y, Ohara S, Ishihara K, Okabe H, Hotta K
Gut. 1980 Jun;21(6):533-6. doi: 10.1136/gut.21.6.533.
Quantitative changes of gastric mucosal glycoproteins with the gastric damage induced by acetylsalicylic acid (aspirin) in rat have been studied. Gastric injury was easily observed macroscopically within one hour after the oral administration of aspirin. The most striking changes occurred at five hours, and the injury was overcome within nine hours after dosing. The glycoproteins extracted from rat stomack with Tris buffer containing Triton X-100 were fractionated on Bio-Gel A-1.5 m column chromatography and divided into three fractions. The first peak, corresponding to gastric mucus macromolecular neutral and acidic glycoproteins with or without sulphate (Fr.I), was diminished after aspirin administration. A considerable alteration of Fr.I (49% of control) appeared at three hours, and a gradual return to the control value was observed subsequently. The changes in the amount of the glycoproteins were detected before the macroscopical changes of the mucosa. These results suggest that gastric ulceration induced by aspirin may be caused by a deficiency of gastric mucus macromolecular glycoproteins of gastric mucus.
研究了大鼠因乙酰水杨酸（阿司匹林）诱导的胃损伤而导致的胃黏膜糖蛋白的定量变化。口服阿司匹林后一小时内，肉眼很容易观察到胃损伤。最显著的变化发生在五小时，给药后九小时内损伤得到缓解。用含有 Triton X - 100 的 Tris 缓冲液从大鼠胃中提取的糖蛋白在 Bio - Gel A - 1.5 m 柱色谱上进行分级分离，分为三个部分。第一个峰对应于含或不含硫酸盐的胃黏液大分子中性和酸性糖蛋白（Fr.I），阿司匹林给药后该峰减少。Fr.I（对照的 49%）在三小时时出现显著变化，随后观察到逐渐恢复到对照值。在黏膜出现肉眼可见变化之前就检测到了糖蛋白量的变化。这些结果表明，阿司匹林诱导的胃溃疡可能是由胃黏液大分子糖蛋白缺乏引起的。
