Embley T M, Finlay B J, Brown S
Department of Zoology, Natural History Museum, London, UK.
FEMS Microbiol Lett. 1992 Oct 1;76(1-2):57-61. doi: 10.1016/0378-1097(92)90363-s.
The polymerase chain reaction was used to amplify and partially sequence the 16S ribosomal RNA genes of symbiotic bacteria within the anaerobic ciliate Metopus contortus. In situ probing with fluorescent oligonucleotides showed that the amplified sequences originated from a single species of archaebacterium which is closely related to Methanocorpusculum parvum. The probed symbionts exhibited a variety of shapes and sizes. These data support the hypothesis, first proposed on the basis of electron microscopy, that the symbionts undergo a morphological transformation as part of the symbiotic process.
采用聚合酶链反应扩增了厌氧纤毛虫扭曲变形虫（Metopus contortus）内共生细菌的16S核糖体RNA基因，并对其进行了部分测序。用荧光寡核苷酸进行原位探测表明，扩增序列源自一种与微小甲烷微菌（Methanocorpusculum parvum）密切相关的古细菌。探测到的共生体呈现出多种形状和大小。这些数据支持了最初基于电子显微镜提出的假说，即共生体作为共生过程的一部分会经历形态转变。
