Omar Atef, Zhang Qianqian, Zou Songbao, Gong Jun
Laboratory of Microbial Ecology and Matter Cycles, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Department of Zoology, Al-Azhar University, Assiut, 71524, Egypt.
J Eukaryot Microbiol. 2017 Nov;64(6):792-805. doi: 10.1111/jeu.12411. Epub 2017 Apr 10.
The morphology and infraciliature of a new ciliate, Metopus yantaiensis n. sp., discovered in coastal soil of northern China, were investigated. It is distinguished from its congeners by a combination of the following features: nuclear apparatus situated in the preoral dome; 18-21 somatic ciliary rows, of which three extend onto the preoral dome (dome kineties); three to five distinctly elongated caudal cilia, and 21-29 adoral polykinetids. The 18S rRNA genes of this new species and two congeners, Metopus contortus and Metopus hasei, were sequenced and phylogenetically analyzed. The new species is more closely related to M. hasei and the clevelandellids than to other congeners; both the genus Metopus and the order Metopida are not monophyletic. In addition, the digestion-resistant bacteria in the cytoplasm of M. yantaiensis were identified, using a 16S rRNA gene clone library, sequencing, and fluorescence in situ hybridization. The detected intracellular bacteria are affiliated with Sphingomonadales, Rhizobiales, Rickettsiales (Alphaproteobacteria), Pseudomonas (Gammaproteobacteria), Rhodocyclales (Betaproteobacteria), Clostridiales (Firmicutes), and Flavobacteriales (Bacteroidetes).
对在中国北方沿海土壤中发现的一种新的纤毛虫——烟台梅托普斯（Metopus yantaiensis n. sp.）的形态和纤毛下器进行了研究。它与同属其他物种的区别在于具有以下特征组合：核器位于口前穹顶；18 - 21列体纤毛，其中3列延伸至口前穹顶（穹顶动纤列）；3 - 5根明显细长的尾纤毛，以及21 - 29个口侧多动基体列。对该新物种以及两个同属物种——扭曲梅托普斯（Metopus contortus）和长泽梅托普斯（Metopus hasei）的18S rRNA基因进行了测序和系统发育分析。该新物种与长泽梅托普斯以及克利夫兰虫类的亲缘关系比与其他同属物种更近；梅托普斯属和梅托普斯目都不是单系的。此外，利用16S rRNA基因克隆文库、测序和荧光原位杂交技术，对烟台梅托普斯细胞质中的抗消化细菌进行了鉴定。检测到的细胞内细菌隶属于鞘脂单胞菌目、根瘤菌目、立克次氏体目（α - 变形菌纲）、假单胞菌属（γ - 变形菌纲）、红环菌目（β - 变形菌纲）、梭菌目（厚壁菌门）和黄杆菌目（拟杆菌门）。
