Fenchel T, Ramsing N B
Marine Biological Laboratory, University of Copenhagen, Helsingør, Denmark.
Arch Microbiol. 1992;158(6):394-7. doi: 10.1007/BF00276298.
The identity of ectosymbiotic bacteria of some marine, free-living anaerobic ciliates (Metopus contortus, Caenomorpha levanderi and Parablepharisma sp.) was studied using fluorescent-dye-conjugated oligonucleotides complementary to short sequence elements of 16S ribosomal RNA. The ectosymbiotic bacteria of all species hybridized with a eubacterial probe and those of the two former mentioned species hybridized with a general probe for sulphate-reducing bacteria, but not to a probe specific for Desulfobacter. The results support indirect evidence suggesting that ectosymbiotic bacteria of anaerobic ciliates are sulphate-reducers which depend on host metabolites for substrates.
利用与16S核糖体RNA短序列元件互补的荧光染料偶联寡核苷酸，研究了一些海洋自由生活厌氧纤毛虫（扭曲后口虫、莱氏海毛虫和拟双小核草履虫）的外共生细菌的特性。所有物种的外共生细菌均与一种真细菌探针杂交，上述前两个物种的外共生细菌与一种针对硫酸盐还原细菌的通用探针杂交，但不与脱硫杆菌特异性探针杂交。这些结果支持了间接证据，表明厌氧纤毛虫的外共生细菌是硫酸盐还原菌，它们依赖宿主代谢产物作为底物。
