Tezak Z, Simić B, Kniewald J
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia.
Food Chem Toxicol. 1992 Oct;30(10):879-85. doi: 10.1016/0278-6915(92)90054-o.
The effect of gamma-1,2,3,4,5,6-hexachlorocyclohexane (lindane), 2-chloro-4-ethylamino-6-isopropylamino-s-triazine (atrazine) and 2-methylthio-4,6-bis-isopropylamino-s-triazine (prometryne) on the formation of a specific oestradiol-receptor complex in the rat uterus cytosol has been examined in vitro and in vivo. Both in vitro and in vivo, the pesticides significantly (P < 0.001) inhibited the formation of the complex in the rat uterus cytosol. The decrease in the number of free specific binding sites on the receptors was determined. The affinity of binding was not modified under the influence of pesticides, and the Kd value was of the same order of magnitude (10(-9) M). The inhibition was found to be fully non-competitive.
已在体外和体内研究了γ-1,2,3,4,5,6-六氯环己烷（林丹）、2-氯-4-乙氨基-6-异丙氨基-s-三嗪（莠去津）和2-甲硫基-4,6-双异丙氨基-s-三嗪（扑草净）对大鼠子宫胞质溶胶中特异性雌二醇受体复合物形成的影响。无论是在体外还是体内，这些农药均显著（P < 0.001）抑制大鼠子宫胞质溶胶中复合物的形成。测定了受体上游离特异性结合位点数量的减少情况。在农药的影响下，结合亲和力未发生改变，解离常数（Kd）值处于相同数量级（10⁻⁹ M）。发现这种抑制作用完全是非竞争性的。
