Garai J, Vértes M, Kovács S
Department of Pathophysiology, University Medical School Pécs, Hungary.
J Steroid Biochem. 1989 Mar;32(3):433-8. doi: 10.1016/0022-4731(89)90218-5.
The effect of cytosolic ultrafiltrates prepared from intact rat uteri, brain hemispheres and hypothalami and of some opiate analogues on oestradiol binding to nuclear type II sites in rat uterus and hypothalamus was studied. Opiate binding in nuclear fraction of rat uteri was also evaluated. Both uterine and hypothalamic low affinity nuclear oestradiol binding was inhibited by filtrate from uteri, while only hypothalamic nuclear binding was decreased in presence of hypothalamic filtrate. Filtrate from brain was ineffective on nuclear oestradiol binding of the studied tissues. Concentration dependent inhibition of uterine nuclear oestradiol binding could be demonstrated by some opiate analogues in vitro. Specific low affinity nuclear binding of opiate antagonist naloxone and agonist dihydromorphine was observed in rat uteri which could be inhibited by uterine filtrate and oestradiol but not by hypothalamic filtrate or other steroids. Present findings support the probable intracellular interplay of opiates and oestradiol action and suggest that cytosolic inhibitor factor might be involved.
研究了从完整大鼠子宫、脑半球和下丘脑制备的胞质超滤液以及一些阿片类似物对大鼠子宫和下丘脑核II型位点雌二醇结合的影响。还评估了大鼠子宫核部分中的阿片结合情况。子宫滤液抑制了子宫和下丘脑低亲和力核雌二醇结合，而下丘脑滤液存在时仅下丘脑核结合减少。脑滤液对所研究组织的核雌二醇结合无效。一些阿片类似物在体外可证明对子宫核雌二醇结合有浓度依赖性抑制作用。在大鼠子宫中观察到阿片拮抗剂纳洛酮和激动剂二氢吗啡的特异性低亲和力核结合，其可被子宫滤液和雌二醇抑制，但不能被下丘脑滤液或其他类固醇抑制。目前的研究结果支持阿片类药物与雌二醇作用可能存在细胞内相互作用，并表明可能涉及胞质抑制因子。
