Wang X, Ray K, Szpirer J, Levan G, Liebhaber S A, Cooke N E
Department of Medicine, Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia 19104.
Genomics. 1992 Oct;14(2):391-7. doi: 10.1016/s0888-7543(05)80231-9.
The human cysteine-rich protein (hCRP) is encoded by a highly conserved and widely expressed serum-inducible immediate early response gene. hCRP contains two copies of the "LIM/double zinc-finger" motif. Using a characterized hCRP cDNA probe, we demonstrate that the human CRP gene (CSRP) is present in a single copy and that both mouse and human genomes contain one or more CRP-related genes detected by hybridization at low stringency. Using a panel of human x rodent somatic cell hybrids, the hCRP locus is assigned to chromosome 1. In situ hybridization of 3H-labeled CRP cDNA to human metaphase chromosomes confirms this assignment and permits regional localization to bands 1q24-1q32. A common MspI polymorphism is identified and mapped to intron 4 of the hCRP gene. The chromosomal localization and restriction site polymorphism should prove useful in future studies of the function of this gene.
人类富含半胱氨酸蛋白（hCRP）由一个高度保守且广泛表达的血清诱导型即刻早期反应基因编码。hCRP含有两个“LIM/双锌指”基序拷贝。使用一个经过鉴定的hCRP cDNA探针，我们证明人类CRP基因（CSRP）以单拷贝形式存在，并且小鼠和人类基因组均包含一个或多个通过低严谨度杂交检测到的CRP相关基因。利用一组人 - 啮齿动物体细胞杂种，将hCRP基因座定位于1号染色体。用3H标记的CRP cDNA对人类中期染色体进行原位杂交证实了这一定位，并允许将其区域定位于1q24 - 1q32带。鉴定出一个常见的MspI多态性并将其定位到hCRP基因的内含子4。染色体定位和限制性酶切位点多态性在该基因功能的未来研究中应会证明是有用的。
