Kishimoto J, Spurr N, Liao M, Lizhi L, Emson P, Xu W
Department of Neurobiology, AFRC, Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, United Kingdom.
Genomics. 1992 Nov;14(3):802-4. doi: 10.1016/s0888-7543(05)80192-2.
Recent research has shown that nitric oxide is a novel neuronal second messenger and transmitter that may be involved in neuronal cell death and damage in neurological illness. To map the chromosomal localization of this important brain enzyme, a rat cDNA probe was prepared by RNA PCR from rat cerebellum RNA. This rat cDNA was used to isolate a human nitric oxide synthase (NOS) cDNA from a human cerebellum cDNA library. The human cDNA clone containing 1.2 kb of brain NOS cDNA was hybridized to Southern blots containing DNAs obtained from human-rodent hybrid cell line panels using EcoRI and HindIII digestion to ascertain the location of the human NOS gene. These data showed that the human brain nitric oxide synthase mapped within 12q14-qter on human chromosome 12.
最近的研究表明，一氧化氮是一种新型的神经元第二信使和递质，可能参与神经疾病中的神经元细胞死亡和损伤。为了确定这种重要脑酶的染色体定位，通过RNA PCR从大鼠小脑RNA制备了大鼠cDNA探针。该大鼠cDNA用于从人小脑cDNA文库中分离人一氧化氮合酶（NOS）cDNA。将含有1.2 kb脑NOS cDNA的人cDNA克隆与含有从人-啮齿动物杂交细胞系面板获得的DNA的Southern印迹杂交，使用EcoRI和HindIII消化以确定人NOS基因的位置。这些数据表明，人脑中的一氧化氮合酶定位于人类第12号染色体的12q14-qter区域内。
