Schijman A G, Levitus G, Levin M J
Instituto de Investigaciones en Ingeniería Genética y Biologia Molecular, Buenos Aires, Argentina.
Immunol Lett. 1992 Jun;33(1):15-20. doi: 10.1016/0165-2478(92)90087-5.
A Trypanosoma cruzi cDNA lambda gt11 recombinant, C-P0, encoding the carboxyterminus of a highly antigenic ribosomal P protein, was isolated. Sequence comparisons and immunological evidence allowed its identification as the C-terminal region of the T. cruzi 38-kDa ribosomal P0 protein. This recombinant failed to react with systemic lupus erythematosus (SLE) anti-P antibodies. The T. cruzi ribosomal P0 protein is the first reported eukaryotic ribosomal P protein presenting this immunological property, probably due to its peculiar C-terminal-amino-acid sequence, FGMGALF, which differs from the conserved eukaryotic ribosomal P protein C-terminal consensus, MGFGLFD.
分离出一种克氏锥虫cDNAλgt11重组体C-P0，它编码一种高度抗原性核糖体P蛋白的羧基末端。序列比较和免疫学证据表明它是克氏锥虫38 kDa核糖体P0蛋白的C末端区域。该重组体与系统性红斑狼疮（SLE）抗P抗体不发生反应。克氏锥虫核糖体P0蛋白是首个报道具有这种免疫学特性的真核核糖体P蛋白，这可能归因于其独特的C末端氨基酸序列FGMGALF，它不同于保守的真核核糖体P蛋白C末端共有序列MGFGLFD。
