ARONSON J
J Cell Biol. 1962 Apr;13(1):33-41. doi: 10.1083/jcb.13.1.33.
Myofibrils which lengthen by several per cent in the presence of ATP and magnesium ions were prepared by teasing indirect flight muscle of Drosophila in solutions containing ethylenediaminetetraacetate. A study was made of the hydrogen ion, magnesium ion, ATP, and potassium chloride concentrations with which this effect could be observed. The lack of elongation with pyrophosphate and several nucleoside triphosphates suggests that the lengthening is ATP specific. A relaxing factor system comparable to that described for rabbit muscle was not demonstrable, as elongated fibrils did not shorten with calcium ions, carnosine, or digitonin.
在含有乙二胺四乙酸的溶液中对果蝇间接飞行肌进行剥离,制备出在三磷酸腺苷(ATP)和镁离子存在下可伸长百分之几的肌原纤维。研究了能观察到这种效应的氢离子、镁离子、ATP和氯化钾浓度。焦磷酸和几种核苷三磷酸不能使肌原纤维伸长,这表明伸长作用具有ATP特异性。未证明存在与兔肌中所描述的类似的舒张因子系统,因为伸长的肌原纤维不会因钙离子、肌肽或洋地黄皂苷而缩短。