BACH J A, SADOFF H L
J Bacteriol. 1962 Apr;83(4):699-707. doi: 10.1128/jb.83.4.699-707.1962.
Bach, John A. (Michigan State University, East Lansing) and H. L. Sadoff. Aerobic sporulating bacteria. I. Glucose dehydrogenase of Bacillus cereus. J. Bacteriol. 83:699-707. 1962.-A heat-resistant glucose dehydrogenase occurs in cultures of Bacillus cereus which are in the initial stages of sporulation. This enzyme is, kinetically, identical to the glucose dehydrogenase which can be extracted from mature spores, but is considerably more heat resistant than the spore-free enzyme. The two enzymes produce identity lines in two-dimensional immunodiffusion experiments, and their behavior in chromatographic and electrophoretic studies is also identical.A labile glucose dehydrogenase can be extracted from germinated spores of B. cereus. It differs from its stable counterparts in possessing a higher pH optimum for enzymatic activity.
巴赫，约翰·A.（密歇根州立大学，东兰辛）和H.L.萨多夫。需氧芽孢杆菌。I.蜡样芽孢杆菌的葡萄糖脱氢酶。《细菌学杂志》83:699 - 707。1962年。——在处于芽孢形成初始阶段的蜡样芽孢杆菌培养物中存在一种耐热的葡萄糖脱氢酶。从动力学角度来看，这种酶与可从成熟芽孢中提取的葡萄糖脱氢酶相同，但比无芽孢的酶耐热性强得多。在二维免疫扩散实验中，这两种酶产生相同的沉淀线，并且它们在色谱和电泳研究中的行为也相同。一种不稳定的葡萄糖脱氢酶可从蜡样芽孢杆菌的萌发芽孢中提取。它与稳定的对应物不同，其酶活性的最适pH值更高。
