Sorensen V, Barfod K, Feld N C
Federation of Danish Pig Producers and Slaughterhouses, Veterinary Department Laboratory, Roskilde.
Vet Rec. 1992 May 30;130(22):488-90. doi: 10.1136/vr.130.22.488.
A monoclonal blocking enzyme-linked immunosorbent assay (ELISA) and an indirect haemagglutination assay (IHA) were applied to serum samples from 124 specific pathogen-free (SPF) breeding and multiplying herds, which participate in the routine serological surveillance of the Danish SPF programme. Clinical and pathological observations of the herds and microbiological culturing of Mycoplasma hyopneumoniae were used to calculate herd sensitivity, herd specificity and herd predictive values for the two serological assays. The ELISA was superior to the IHA in herd sensitivity and herd specificity, with values of 93 per cent and 96 per cent, respectively, for the ELISA, and 61 per cent and 92 per cent for the IHA. During the six month period of evaluation 2.5 per cent of the herds were infected with M hyopneumoniae each month. At this level the IHA was found to have a positive herd predictive value of 16 per cent, compared with 39 per cent for the ELISA. The negative herd-predictive value on the same level was 99.8 per cent for the ELISA and 98.9 per cent for the IHA. If the assays were applied to a group of herds with a herd prevalence of M hyopneumoniae infection of 30 per cent (as is the case with the production herds in the Danish SPF programme) the predictive value of a positive herd diagnosis would be 91 per cent for the ELISA and 76 per cent for the IHA, and the predictive value of a negative herd diagnosis would be 97 per cent with the ELISA and 85 per cent with the IHA.(ABSTRACT TRUNCATED AT 250 WORDS)
采用单克隆阻断酶联免疫吸附测定法（ELISA）和间接血凝试验（IHA），对参与丹麦无特定病原体（SPF）计划常规血清学监测的124个SPF繁殖和扩繁畜群的血清样本进行检测。利用畜群的临床和病理观察结果以及猪肺炎支原体的微生物培养，计算这两种血清学检测方法的畜群敏感性、畜群特异性和畜群预测值。ELISA在畜群敏感性和畜群特异性方面优于IHA，ELISA的值分别为93%和96%，IHA的值分别为61%和92%。在六个月的评估期内，每月有2.5%的畜群感染猪肺炎支原体。在此感染水平下，IHA的阳性畜群预测值为16%，而ELISA为39%。ELISA的阴性畜群预测值在同一感染水平下为99.8%，IHA为98.9%。如果将这些检测方法应用于猪肺炎支原体感染畜群患病率为30%的一组畜群（丹麦SPF计划中的生产畜群就是这种情况），ELISA的阳性畜群诊断预测值将为91%，IHA为76%，ELISA的阴性畜群诊断预测值为97%，IHA为85%。（摘要截选至250词）
