Jeannin P, Didierlaurent A, Gras-Masse H, Elass A A, Delneste Y, Cardot E, Joseph M, Tartar A, Vergoten G, Pestel J
Laboratoire de pathologie immunoallergique respiratoire et cellules inflammatoires, Institut Pasteur, Lille, France.
Mol Immunol. 1992 Jun;29(6):739-49. doi: 10.1016/0161-5890(92)90184-y.
Dust mite allergens are considered as a major cause of allergic disease and as a risk factor for asthma. Der p I, a 222 amino-acid residue globular glycoprotein, is one of the major allergens from Dermatophagoides pteronyssinus (Dpt) mites. In this study, we have used predictive conventional algorithms (i.e. hydrophilicity, mobility, accessibility) and a three-dimensional model of Der p I derived from comparison to actinidin and papain to select continuous amino acid sequences as potential B cell epitopes. Four peptides, 52-71, 117-133, 176-187, 188-199 were synthesized. Their antigenic reactivity was investigated, mainly by measuring their capacity to induce in vitro histamine release. Results indicated that only Dpt-sensitive patients react specifically to Der p I-derived peptides and more frequently to 52-71 and 117-133. For each peptide, the intensity of response was dependent on the patient tested and on the peptide concn. The capacity of peptides to induce histamine release was demonstrated to be correlated with the serum level of anti-Der p I IgE (r = 0.86; p less than 10(-2)). Taken together these data emphasize, in Dpt-sensitive patients, the heterogeneity of the specific response to synthetic Der p I-derived peptides and underline the possible variety of epitopes belonging to the allergen Der p I.
尘螨过敏原被认为是过敏性疾病的主要病因和哮喘的危险因素。Der p I是一种由222个氨基酸残基组成的球状糖蛋白，是来自粉尘螨的主要过敏原之一。在本研究中，我们使用了预测性传统算法（即亲水性、迁移率、可及性）以及通过与肌动蛋白水解酶和木瓜蛋白酶比较得出的Der p I三维模型，来选择连续氨基酸序列作为潜在的B细胞表位。合成了四条肽段，分别为52 - 71、117 - 133、176 - 187、188 - 199。主要通过测量它们诱导体外组胺释放的能力来研究其抗原反应性。结果表明，只有对粉尘螨敏感的患者对Der p I衍生肽有特异性反应，且对52 - 71和117 - 133的反应更频繁。对于每条肽段，反应强度取决于所测试的患者和肽段浓度。已证明肽段诱导组胺释放的能力与抗Der p I IgE的血清水平相关（r = 0.86；p < 10⁻²）。综合这些数据强调，在对粉尘螨敏感的患者中，对合成的Der p I衍生肽的特异性反应存在异质性，并突显了属于过敏原Der p I的表位可能具有多样性。
