Lunardi C, Marguerie C, Walport M J, So A K
Rheumatology Unit, Royal Postgraduate Medical School, London.
Br J Rheumatol. 1992 Aug;31(8):527-30. doi: 10.1093/rheumatology/31.8.527.
We have determined the distribution of T gamma delta cells in the peripheral blood of 44 patients with rheumatoid arthritis and in 36 healthy controls. In addition, paired blood and synovial fluid samples were obtained from seven patients with RA. The monoclonal antibodies A13, BB3 and Ti gamma A, which are specific for the V delta 1, V delta 2 and V gamma 9 gene products respectively, were used to define T gamma delta subsets. T gamma delta + cells expressed as a percentage of CD3+ lymphocytes were reduced in RA peripheral blood compared with the control group (3.9% +/- 0.5 versus 5.7% +/- 0.7; P less than 0.0001). There was a reduction in the V gamma 9/V delta 2+ subset (from 5.6% +/- 1.2 to 1.7% +/- 0.4) leading to a change in the mean ratio of V delta 2/V delta 1+ cells from 4.3 in normal subjects to 1.1 (P less than 0.002). No statistical difference was observed in T gamma delta cell numbers in synovial fluid compared with the paired blood samples (4.0% +/- 1.1 in blood and 4.4% +/- 1.4 in synovial fluid). Also the distribution of V delta 2+ and V delta 1+ cells was similar in the two compartments and a similar alteration in subset distribution was found in blood and synovial fluid. These findings do not indicate a selective accumulation of a specific T gamma delta subset in RA synovial effusions.
我们已经确定了44例类风湿性关节炎患者及36名健康对照者外周血中Tγδ细胞的分布情况。此外，还从7例类风湿性关节炎患者身上获取了配对的血液和滑液样本。分别针对Vδ1、Vδ2和Vγ9基因产物的单克隆抗体A13、BB3和TiγA，用于界定Tγδ亚群。与对照组相比，类风湿性关节炎患者外周血中Tγδ +细胞占CD3 +淋巴细胞的百分比降低（3.9%±0.5对5.7%±0.7；P＜0.0001）。Vγ9/Vδ2 +亚群减少（从5.6%±1.2降至1.7%±0.4），导致Vδ2/Vδ1 +细胞的平均比例从正常受试者的4.3变为1.1（P＜0.002）。与配对的血液样本相比，滑液中Tγδ细胞数量未观察到统计学差异（血液中为4.0%±1.1，滑液中为4.4%±1.4）。而且，Vδ2 +和Vδ1 +细胞在两个腔室中的分布相似，血液和滑液中的亚群分布也有类似变化。这些发现并未表明类风湿性关节炎滑膜积液中有特定Tγδ亚群的选择性积聚。
