Shen Y, Li S, Quayle A J, Mellbye O J, Natvig J B, Førre O
Institute of Immunology and Rheumatology, National Hospital, Oslo, Norway.
Scand J Immunol. 1992 Oct;36(4):533-40. doi: 10.1111/j.1365-3083.1992.tb03221.x.
Using a peroxidase/anti-peroxidase immunohistochemical staining method, we examined sections of inflammatory synovial membranes from 13 patients with juvenile rheumatoid arthritis (JRA) and 11 with rheumatoid arthritis (RA). The relative numbers of TCR gamma/delta+ cells and the proportions of V delta 1+ and V delta 2+ subsets were recorded in the areas of the membranes most heavily infiltrated by CD3+ cells. In the JRA group, the majority (8/13) of the membranes had TCR gamma/delta+ cells which contributed between 5 and less than 10% of the total number of CD3+ cells. In the RA synovial membranes examined, 5/11 samples had between 5 and 10% TCR gamma/delta+ cells, but in another 5 TCR gamma/delta+ cells contributed to between 10 and 20% of CD3+ cells. No significant difference was noted between the two patient groups. However, the range of values found in the RA membranes appeared to be slightly higher in comparison to previously reported values for RA synovial fluid, peripheral blood and eluted synovial membrane T cells. Analysis of the relative proportions of the V delta 1+ and V delta 2+ subsets revealed a significant dominance of V delta 1+ cells in RA membranes and approximately equal numbers of the two populations in the JRA patients. As the majority of peripheral blood TCR gamma/delta+ cells use the V delta 2 segment this suggests a preferential homing or expansion of the V delta 1+ cells in both RA and JRA synovium. The overall distribution pattern of the TCR gamma/delta+ and V delta 1+ and V delta 2+ cells was also recorded. These cells mostly accumulated in the lymphoid-like tissues and in the perivascular area in the tissues of both RA and JRA patients. Occasionally, augmented numbers of these cells were found in the subsynovial layer or in the loose connective tissue. In the majority of cases, only a few TCR gamma/delta+ cells were located in the synovial layer. The function and the possible pathogenetic importance of these TCR gamma/delta+ cells have not so far been determined.
我们采用过氧化物酶/抗过氧化物酶免疫组化染色方法，检查了13例幼年类风湿性关节炎（JRA）患者和11例类风湿性关节炎（RA）患者的炎性滑膜组织切片。在CD3 +细胞浸润最严重的滑膜区域，记录TCRγ/δ +细胞的相对数量以及Vδ1 +和Vδ2 +亚群的比例。在JRA组中，大多数（8/13）滑膜组织有TCRγ/δ +细胞，其占CD3 +细胞总数的5%至不到10%。在所检查的RA滑膜组织中，5/11的样本有5%至10%的TCRγ/δ +细胞，但另外5个样本中TCRγ/δ +细胞占CD3 +细胞的10%至20%。两组患者之间未发现显著差异。然而，与先前报道的RA滑液、外周血和洗脱的滑膜T细胞的值相比，RA滑膜组织中发现的值范围似乎略高。对Vδ1 +和Vδ2 +亚群相对比例的分析显示，RA滑膜组织中Vδ1 +细胞明显占优势，而JRA患者中这两个群体的数量大致相等。由于大多数外周血TCRγ/δ +细胞使用Vδ2片段，这表明Vδ1 +细胞在RA和JRA滑膜中优先归巢或扩增。还记录了TCRγ/δ +、Vδ1 +和Vδ2 +细胞的总体分布模式。这些细胞大多积聚在RA和JRA患者组织中的类淋巴样组织和血管周围区域。偶尔，在滑膜下层或疏松结缔组织中发现这些细胞数量增加。在大多数情况下，滑膜层中仅发现少数TCRγ/δ +细胞。迄今为止，这些TCRγ/δ +细胞的功能及其可能的致病重要性尚未确定。
