Stringer P, Vintiner S K, Stowell L I, Thomson D G
Criminalistics and Forensic Biology Section, Department of Scientific and Industrial Research, Petone, Wellington, New Zealand.
J Forensic Sci Soc. 1992 Apr-Jun;32(2):101-15. doi: 10.1016/s0015-7368(92)73060-6.
The isoenzymes erythrocyte acid phosphatase and phosphoglucomutase were typed in mixed red cell samples which had been derived from two individuals; the protein group specific component was typed in mixed serum samples. Typing was performed by isoelectric focusing on ultrathin polyacrylamide gels. Depending upon the mixture, from 2 to 20% (but typically 5-10%) by volume of a second blood or serum needed to be present in a mixture before it could be detected. In the majority of cases when there was significant mixing, samples were readily identified as a mixture when the results consisted of unusual band patterns or unusual band intensities. There would be a few instances when blood or serum could not be identified as a mixture when masking effects occurred or when the mixture produced a combined, apparently normal, pattern.
对来自两个人的混合红细胞样本进行了红细胞酸性磷酸酶和磷酸葡萄糖变位酶同工酶分型；对混合血清样本进行了蛋白质组特异性成分分型。分型是通过在超薄聚丙烯酰胺凝胶上进行等电聚焦来完成的。根据混合物的不同，在混合物中需要存在2%至20%（但通常为5%-10%）体积的第二种血液或血清才能被检测到。在大多数有明显混合的情况下，当结果呈现异常的条带模式或异常的条带强度时，样本很容易被识别为混合物。当出现掩盖效应或混合物产生组合的、明显正常的模式时，会有少数情况下血液或血清无法被识别为混合物。
