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用于血迹常规筛查的五种电泳表型系统的评估

The evaluation of five electrophoretic phenotyping systems for routine screening of bloodstains.

作者信息

Thomas A S

出版信息

J Forensic Sci Soc. 1989 Jul-Aug;29(4):243-8. doi: 10.1016/s0015-7368(89)73258-8.

DOI:10.1016/s0015-7368(89)73258-8
PMID:2529346
Abstract

The performance of the polymorphic marker systems group-specific component (GC), phosphoglucomutase-1 (PGM), alpha-2-HS-glycoprotein (A2HS), haptoglobin (Hp), and erythrocyte acid phosphatase (EAP) was evaluated on control bloodstains. The major factors considered were: sensitivity of the test system; stability of the marker; laboratory economics of each test; and distinguishing power (Dp) of the system. GC was considered to be the most suitable marker for routine screening because of its high stability and Dp, and the sensitivity of the immunoblotting detection method. PGM and A2HS were the next most valuable markers followed by Hp. EAP could only be considered useful when large amounts of relatively fresh bloodstain were available.

摘要

在对照血迹上评估了多态性标记系统,即组特异性成分(GC)、磷酸葡萄糖变位酶-1(PGM)、α-2-HS-糖蛋白(A2HS)、触珠蛋白(Hp)和红细胞酸性磷酸酶(EAP)的性能。所考虑的主要因素包括:检测系统的灵敏度;标记物的稳定性;每项检测的实验室经济性;以及该系统的鉴别能力(Dp)。由于GC具有高稳定性和鉴别能力,以及免疫印迹检测方法的灵敏度,因此被认为是常规筛查最适合的标记物。PGM和A2HS是其次最有价值的标记物,其次是Hp。只有在有大量相对新鲜的血迹时,EAP才被认为是有用的。

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