Kellerman S A, Hunter A G
Department of Animal Science, University of Minnesota, St. Paul 55108.
J Dairy Sci. 1992 Jun;75(6):1443-7. doi: 10.3168/jds.S0022-0302(92)77899-0.
In order to investigate whether Ig molecules affect bovine fertility, bovine follicular oocytes were tested for an oolemmal receptor that recognized IgG. The zona pellucida was removed from 296 oocytes. The zona-free oocytes were then incubated with purified bovine IgG. its Fc fragment, or its Fab fragment. They were subsequently washed and incubated with the appropriate rabbit antibovine IgG (whole IgG-specific, Fc-specific, or Fab-specific), which was covalently attached to polyacrylamide beads (immunobeads). Bead attachment was scored using light microscopy. Bovine IgG and bovine IgG-Fc bound to the oocytes, whereas the IgG-Fab exhibited negligible binding. The data indicate that a bovine oolemmal receptor exists that binds IgG by recognizing the molecule in the region of the Fc-Fab junction. Thus, the binding of IgG or other Ig molecules to bovine oocytes may play a role in sperm and egg interaction.
为了研究免疫球蛋白（Ig）分子是否影响牛的生育能力，对牛卵泡卵母细胞进行检测，以寻找一种能识别IgG的卵膜受体。从296个卵母细胞上去除透明带。然后将无透明带的卵母细胞与纯化的牛IgG、其Fc片段或Fab片段一起孵育。随后对它们进行洗涤，并与共价连接到聚丙烯酰胺珠（免疫珠）上的相应兔抗牛IgG（全IgG特异性、Fc特异性或Fab特异性）一起孵育。使用光学显微镜对珠附着情况进行评分。牛IgG和牛IgG-Fc与卵母细胞结合，而IgG-Fab的结合可忽略不计。数据表明存在一种牛卵膜受体，该受体通过识别Fc-Fab连接区域的分子来结合IgG。因此，IgG或其他Ig分子与牛卵母细胞的结合可能在精子与卵子的相互作用中发挥作用。
